miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1301 3' -50.8 NC_001331.1 + 1089 0.68 0.2621
Target:  5'- gUUGGggGcguucUUCGCCGGcgCCuucaucaccgGGUACCg -3'
miRNA:   3'- -AAUCuuCuu---AAGCGGCCa-GG----------CCAUGG- -5'
1301 3' -50.8 NC_001331.1 + 1157 1.09 0.000158
Target:  5'- gUUAGAAGAAUUCGCCGGUCCGGUACCc -3'
miRNA:   3'- -AAUCUUCUUAAGCGGCCAGGCCAUGG- -5'
1301 3' -50.8 NC_001331.1 + 2256 0.66 0.343151
Target:  5'- gUGGGuuccuuGAGUUCGCCG--CCGGgGCCu -3'
miRNA:   3'- aAUCUu-----CUUAAGCGGCcaGGCCaUGG- -5'
1301 3' -50.8 NC_001331.1 + 4616 0.67 0.305049
Target:  5'- gUGGucGAGUUCGCCGGcuacgaaUGGU-CCa -3'
miRNA:   3'- aAUCuuCUUAAGCGGCCag-----GCCAuGG- -5'
1301 3' -50.8 NC_001331.1 + 6613 0.66 0.363448
Target:  5'- ---------gUCGCCGuGUCCGGacUGCCg -3'
miRNA:   3'- aaucuucuuaAGCGGC-CAGGCC--AUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.