Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13011 | 5' | -52.3 | NC_003390.1 | + | 6885 | 0.66 | 0.792751 |
Target: 5'- gCAGAGaCCGGCuUCAUGaagcaucagGAGGCCg- -3' miRNA: 3'- aGUUUC-GGUCGuAGUACg--------UUCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 6932 | 0.66 | 0.792751 |
Target: 5'- aCAGGGCCcguGCAg---GCAAGGCUa- -3' miRNA: 3'- aGUUUCGGu--CGUaguaCGUUCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 18257 | 0.66 | 0.792751 |
Target: 5'- ----cGCCaAGgGUgGUGCGAuGGCCUCc -3' miRNA: 3'- aguuuCGG-UCgUAgUACGUU-CCGGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 36252 | 0.66 | 0.791718 |
Target: 5'- cUCAuAAGCCGGauaagGUCcuugggauaagccGUGCAAGGCCa- -3' miRNA: 3'- -AGU-UUCGGUCg----UAG-------------UACGUUCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 5143 | 0.66 | 0.791718 |
Target: 5'- aUCAGAGCCuucuguuGGUugaugaggCGUGCGAGGUCa- -3' miRNA: 3'- -AGUUUCGG-------UCGua------GUACGUUCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 15141 | 0.66 | 0.781294 |
Target: 5'- cUCAAGGCCacuGGCGUCAcuaccaaccUGCAggugguuaucacuGGGCUUg -3' miRNA: 3'- -AGUUUCGG---UCGUAGU---------ACGU-------------UCCGGAg -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 28400 | 0.66 | 0.761024 |
Target: 5'- gCAGAGCCuucgaccauAGC-UUGUGagAGGGCCUCg -3' miRNA: 3'- aGUUUCGG---------UCGuAGUACg-UUCCGGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 25958 | 0.66 | 0.750136 |
Target: 5'- -aGAAGCCAGCAcgCGaGCAAuGCgCUCg -3' miRNA: 3'- agUUUCGGUCGUa-GUaCGUUcCG-GAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 5709 | 0.67 | 0.739113 |
Target: 5'- cUCAAAGUCAGCAUUgcGgAAGauGCCUUc -3' miRNA: 3'- -AGUUUCGGUCGUAGuaCgUUC--CGGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 44313 | 0.67 | 0.72797 |
Target: 5'- aCGAAGCCAGCAcCA-GCAA--CCUCc -3' miRNA: 3'- aGUUUCGGUCGUaGUaCGUUccGGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 44059 | 0.68 | 0.67092 |
Target: 5'- cUCAAAGCCuGCAUCcacCAGGGCg-- -3' miRNA: 3'- -AGUUUCGGuCGUAGuacGUUCCGgag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 38342 | 0.68 | 0.647724 |
Target: 5'- cUCAAGGCCuGCAggGUGagcaggggcuaCAAGGcCCUCa -3' miRNA: 3'- -AGUUUCGGuCGUagUAC-----------GUUCC-GGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 38302 | 0.68 | 0.636095 |
Target: 5'- -gGGGGCCAGUAUCAccuuggagaccuUG-AGGGCCUUc -3' miRNA: 3'- agUUUCGGUCGUAGU------------ACgUUCCGGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 3724 | 0.68 | 0.636095 |
Target: 5'- gCGAGGCCuuGCAUgAUGCu-GGCUUUg -3' miRNA: 3'- aGUUUCGGu-CGUAgUACGuuCCGGAG- -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 2471 | 0.69 | 0.578118 |
Target: 5'- aCAAGGCUGGCAUCcaGUGgAAGGgCUg -3' miRNA: 3'- aGUUUCGGUCGUAG--UACgUUCCgGAg -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 15302 | 0.7 | 0.532562 |
Target: 5'- -aAGGGCCAagaaauacGUggaaagAUCAUGCAAGGCCUa -3' miRNA: 3'- agUUUCGGU--------CG------UAGUACGUUCCGGAg -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 225 | 0.72 | 0.415538 |
Target: 5'- aUCAGgcAGCgCGGCGUCccuucUGCAGGGCCa- -3' miRNA: 3'- -AGUU--UCG-GUCGUAGu----ACGUUCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 30369 | 0.72 | 0.415538 |
Target: 5'- aUCAGgcAGCgCGGCGUCccuucUGCAGGGCCa- -3' miRNA: 3'- -AGUU--UCG-GUCGUAGu----ACGUUCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 47431 | 0.75 | 0.291746 |
Target: 5'- aCGGAGCCAgGCGUCAcGCucuGGGCCa- -3' miRNA: 3'- aGUUUCGGU-CGUAGUaCGu--UCCGGag -5' |
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13011 | 5' | -52.3 | NC_003390.1 | + | 1850 | 0.76 | 0.2551 |
Target: 5'- ---cAGCCguugcaguauugAGCAaCGUGCAGGGCCUCg -3' miRNA: 3'- aguuUCGG------------UCGUaGUACGUUCCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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