Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13012 | 5' | -52.7 | NC_003390.1 | + | 32499 | 0.66 | 0.767987 |
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Target: 5'- --cCUGCUGCAacCCAGUcacgcggaugUCAUCACGc -3' miRNA: 3'- ccaGACGACGUc-GGUCA----------AGUAGUGUa -5' |
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| 13012 | 5' | -52.7 | NC_003390.1 | + | 44381 | 0.66 | 0.746418 |
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Target: 5'- aGG-UUGCUGguGCUGGcUUCGUCaACAUg -3' miRNA: 3'- -CCaGACGACguCGGUC-AAGUAG-UGUA- -5' |
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| 13012 | 5' | -52.7 | NC_003390.1 | + | 38979 | 0.68 | 0.633016 |
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Target: 5'- aGGaUCUGCUGCuaguGGCCAGggUUAUCuACGc -3' miRNA: 3'- -CC-AGACGACG----UCGGUCa-AGUAG-UGUa -5' |
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| 13012 | 5' | -52.7 | NC_003390.1 | + | 3503 | 0.69 | 0.564108 |
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Target: 5'- uGGUCUGCUuaGCA-CCAGUg-AUCACAc -3' miRNA: 3'- -CCAGACGA--CGUcGGUCAagUAGUGUa -5' |
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| 13012 | 5' | -52.7 | NC_003390.1 | + | 20688 | 0.73 | 0.358041 |
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Target: 5'- cGGcCUGCUGCAGCUugaGGgcagUCAUCugGg -3' miRNA: 3'- -CCaGACGACGUCGG---UCa---AGUAGugUa -5' |
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| 13012 | 5' | -52.7 | NC_003390.1 | + | 3908 | 1.08 | 0.001374 |
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Target: 5'- cGGUCUGCUGCAGCCAGUUCAUCACAUc -3' miRNA: 3'- -CCAGACGACGUCGGUCAAGUAGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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