Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13014 | 3' | -51.2 | NC_003390.1 | + | 20264 | 0.66 | 0.891865 |
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Target: 5'- cCUGGAuGUUGGCUggGAGGGUG-GCAUGg -3' miRNA: 3'- -GACUU-CGACUGG--CUUCCAUaCGUGCg -5' |
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| 13014 | 3' | -51.2 | NC_003390.1 | + | 31857 | 0.67 | 0.83274 |
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Target: 5'- gUGGAGUUGGCCGuuGGUGUcgAgGCc -3' miRNA: 3'- gACUUCGACUGGCuuCCAUAcgUgCG- -5' |
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| 13014 | 3' | -51.2 | NC_003390.1 | + | 32353 | 0.67 | 0.803779 |
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Target: 5'- gUGggGCacggUGACCGAuggagcucuGGGcGUGCAUGa -3' miRNA: 3'- gACuuCG----ACUGGCU---------UCCaUACGUGCg -5' |
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| 13014 | 3' | -51.2 | NC_003390.1 | + | 22158 | 0.67 | 0.803779 |
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Target: 5'- aUGAGGCUGcuaUGAGGGUGU-CugGUa -3' miRNA: 3'- gACUUCGACug-GCUUCCAUAcGugCG- -5' |
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| 13014 | 3' | -51.2 | NC_003390.1 | + | 38918 | 0.68 | 0.762493 |
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Target: 5'- aUGAGuGCUG-CUGAGaGUGUcGCACGCg -3' miRNA: 3'- gACUU-CGACuGGCUUcCAUA-CGUGCG- -5' |
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| 13014 | 3' | -51.2 | NC_003390.1 | + | 42532 | 0.69 | 0.696469 |
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Target: 5'- -aGAAGCgGcCCGGAGGUcgGCGguaguCGCc -3' miRNA: 3'- gaCUUCGaCuGGCUUCCAuaCGU-----GCG- -5' |
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| 13014 | 3' | -51.2 | NC_003390.1 | + | 5729 | 1.12 | 0.00125 |
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Target: 5'- gCUGAAGCUGACCGAAGGUAUGCACGCu -3' miRNA: 3'- -GACUUCGACUGGCUUCCAUACGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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