Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13015 | 3' | -51.6 | NC_003390.1 | + | 33397 | 0.66 | 0.893704 |
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Target: 5'- uCCAu-GGGCuGUUGGGUACcUugGCCu -3' miRNA: 3'- -GGUucUCCGuUAGCUCGUGcAugCGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 38296 | 0.66 | 0.862267 |
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Target: 5'- cCCAGGAGcCGAUgGAGCagACGga-GCCc -3' miRNA: 3'- -GGUUCUCcGUUAgCUCG--UGCaugCGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 17907 | 0.67 | 0.817367 |
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Target: 5'- aCCGAagacGAGGCAGcucUCGuGCGCGaGCaGCUg -3' miRNA: 3'- -GGUU----CUCCGUU---AGCuCGUGCaUG-CGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 14344 | 0.68 | 0.807731 |
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Target: 5'- uCCAuacAGGUuucCGGGCAUGaGCGCCg -3' miRNA: 3'- -GGUuc-UCCGuuaGCUCGUGCaUGCGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 26950 | 0.68 | 0.807731 |
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Target: 5'- aUCAGGAGcUggUCG-GCugGgcCGCCa -3' miRNA: 3'- -GGUUCUCcGuuAGCuCGugCauGCGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 29481 | 0.7 | 0.679267 |
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Target: 5'- aCAGGGGGCcGUgggggauccggagCGGGCGCGggaGCCg -3' miRNA: 3'- gGUUCUCCGuUA-------------GCUCGUGCaugCGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 6863 | 0.7 | 0.66917 |
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Target: 5'- aUCAGGAGGCcGUUGAGCGugcCGguaAUGCUg -3' miRNA: 3'- -GGUUCUCCGuUAGCUCGU---GCa--UGCGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 32462 | 0.73 | 0.481231 |
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Target: 5'- gCAGGaAGGCAAUCGAGgACGcagcugaaGCCg -3' miRNA: 3'- gGUUC-UCCGUUAGCUCgUGCaug-----CGG- -5' |
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| 13015 | 3' | -51.6 | NC_003390.1 | + | 6821 | 1.16 | 0.000812 |
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Target: 5'- cCCAAGAGGCAAUCGAGCACGUACGCCa -3' miRNA: 3'- -GGUUCUCCGUUAGCUCGUGCAUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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