Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13018 | 5' | -61.8 | NC_003390.1 | + | 10127 | 0.66 | 0.333207 |
Target: 5'- aGGuGCAGCuauaaccgagCAGgAGCUCgaUGCCCuCUCc -3' miRNA: 3'- -CCuCGUCG----------GUCgUCGAGg-ACGGG-GAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 27254 | 0.66 | 0.302469 |
Target: 5'- aGGAGUcuugaacuugccAGCCAGgGGCUCa-GCCucagCCUCa -3' miRNA: 3'- -CCUCG------------UCGGUCgUCGAGgaCGG----GGAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 29852 | 0.67 | 0.278791 |
Target: 5'- uGGAGCaugGGCCAGCAGC-CUgugacucugacaagUGUCUCUUc -3' miRNA: 3'- -CCUCG---UCGGUCGUCGaGG--------------ACGGGGAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 19939 | 0.67 | 0.273277 |
Target: 5'- uGGAGCaugGGCCAGCAGC-CUggacucugacaagUGUCUCUUc -3' miRNA: 3'- -CCUCG---UCGGUCGUCGaGG-------------ACGGGGAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 3458 | 0.67 | 0.254024 |
Target: 5'- -aGGCAGCCAGgaacauccaaGGCUCCccaGCaCCCUCc -3' miRNA: 3'- ccUCGUCGGUCg---------UCGAGGa--CG-GGGAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 513 | 0.69 | 0.208838 |
Target: 5'- cGAGCAGCgcaagCAGCAGCUCUucuacuuugcugagaUGgCUCUCa -3' miRNA: 3'- cCUCGUCG-----GUCGUCGAGG---------------ACgGGGAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 5701 | 0.69 | 0.190968 |
Target: 5'- --cGCAGCCAGaCuGC-CCgUGCCCCUa -3' miRNA: 3'- ccuCGUCGGUC-GuCGaGG-ACGGGGAg -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 34150 | 0.69 | 0.181095 |
Target: 5'- -cGGCAGCUAGC-GCUCCccccaugGCCCCc- -3' miRNA: 3'- ccUCGUCGGUCGuCGAGGa------CGGGGag -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 32309 | 0.7 | 0.160088 |
Target: 5'- gGGAGCAGcCCGGCGacaaccacuucguuGUguauugggagaaggUCCUGCCCCa- -3' miRNA: 3'- -CCUCGUC-GGUCGU--------------CG--------------AGGACGGGGag -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 7771 | 0.71 | 0.13825 |
Target: 5'- aGGGGCGgauGCCugAGCAGCUUUaGCCuCCUCg -3' miRNA: 3'- -CCUCGU---CGG--UCGUCGAGGaCGG-GGAG- -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 27221 | 0.73 | 0.100874 |
Target: 5'- aGGAGCAGCCAGaggccucugaggaAGCUCCUGUCg--- -3' miRNA: 3'- -CCUCGUCGGUCg------------UCGAGGACGGggag -5' |
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13018 | 5' | -61.8 | NC_003390.1 | + | 7813 | 1.11 | 0.000106 |
Target: 5'- uGGAGCAGCCAGCAGCUCCUGCCCCUCa -3' miRNA: 3'- -CCUCGUCGGUCGUCGAGGACGGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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