Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13019 | 5' | -49 | NC_003390.1 | + | 2170 | 0.66 | 0.942535 |
Target: 5'- uGGCAAUGCuGcuaAGGGCCGGGGUGg -3' miRNA: 3'- cCUGUUACGuCaagUUCCGGUUCUGC- -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 38249 | 0.67 | 0.919294 |
Target: 5'- aGGGCGA-GCAGggucuaCAGGGCCcacAGGGCc -3' miRNA: 3'- -CCUGUUaCGUCaa----GUUCCGG---UUCUGc -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 38413 | 0.67 | 0.912711 |
Target: 5'- uGACGGUGCuGUUggccccCAAGGCCcagAAGGCc -3' miRNA: 3'- cCUGUUACGuCAA------GUUCCGG---UUCUGc -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 6930 | 0.68 | 0.883352 |
Target: 5'- aGGGCccGUGCAGg-CAAGGCUAcAGAUGc -3' miRNA: 3'- -CCUGu-UACGUCaaGUUCCGGU-UCUGC- -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 31291 | 0.68 | 0.875278 |
Target: 5'- uGACGGUGUAGUuguagcagUCGcAGGCCAGG-Ca -3' miRNA: 3'- cCUGUUACGUCA--------AGU-UCCGGUUCuGc -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 1888 | 0.69 | 0.840252 |
Target: 5'- --cCAGUGgAGggUCGAGGCUGAGGCa -3' miRNA: 3'- ccuGUUACgUCa-AGUUCCGGUUCUGc -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 27539 | 0.69 | 0.840252 |
Target: 5'- cGGACGGUGUAGUcCAcGGUCuucucGGACa -3' miRNA: 3'- -CCUGUUACGUCAaGUuCCGGu----UCUGc -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 36275 | 0.71 | 0.759234 |
Target: 5'- gGGAUAA-GCcGUgCAAGGCC-AGACGa -3' miRNA: 3'- -CCUGUUaCGuCAaGUUCCGGuUCUGC- -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 24624 | 0.72 | 0.708008 |
Target: 5'- gGGACugagcugauuAUGCAGgUCAcuggcauccccaagaGGGCCAAGugGa -3' miRNA: 3'- -CCUGu---------UACGUCaAGU---------------UCCGGUUCugC- -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 15152 | 0.72 | 0.668939 |
Target: 5'- aGACAccGUAGcUCAAGGCCAcuGGCGu -3' miRNA: 3'- cCUGUuaCGUCaAGUUCCGGUu-CUGC- -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 21836 | 0.73 | 0.657341 |
Target: 5'- gGGACAG-GUGG---AGGGCCAGGACGg -3' miRNA: 3'- -CCUGUUaCGUCaagUUCCGGUUCUGC- -5' |
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13019 | 5' | -49 | NC_003390.1 | + | 9154 | 1.13 | 0.00197 |
Target: 5'- uGGACAAUGCAGUUCAAGGCCAAGACGg -3' miRNA: 3'- -CCUGUUACGUCAAGUUCCGGUUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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