Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13021 | 5' | -56.1 | NC_003390.1 | + | 34325 | 0.66 | 0.649012 |
Target: 5'- gCGGCuuGUAG-GGCCCUUAGAaGGCgUUCu -3' miRNA: 3'- -GCUG--CAUCuCCGGGAGUCUgUCG-AGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 43803 | 0.66 | 0.638008 |
Target: 5'- aCGugGUAGAugaguuccuacaGGCCUaUAGAagcGCUCCu -3' miRNA: 3'- -GCugCAUCU------------CCGGGaGUCUgu-CGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 29518 | 0.66 | 0.615993 |
Target: 5'- cCGGCGguggaGGGGGCUcgggCUUAGGCAcacuagguGCUCCu -3' miRNA: 3'- -GCUGCa----UCUCCGG----GAGUCUGU--------CGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 20272 | 0.66 | 0.615993 |
Target: 5'- cCGGCGguggaGGGGGCUcgggCUUAGGCAcacuagguGCUCCu -3' miRNA: 3'- -GCUGCa----UCUCCGG----GAGUCUGU--------CGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 17861 | 0.67 | 0.572204 |
Target: 5'- uGugGUAGAgcgGGCCUcaUCAGGCcuuGCUgCa -3' miRNA: 3'- gCugCAUCU---CCGGG--AGUCUGu--CGAgG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 21856 | 0.67 | 0.55057 |
Target: 5'- ----aUGGuGGCCC-CAGACGGgUCCa -3' miRNA: 3'- gcugcAUCuCCGGGaGUCUGUCgAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 10804 | 0.68 | 0.539846 |
Target: 5'- uCGACGUAGccaAGcGCCgUCGGgGCcaggGGCUCCu -3' miRNA: 3'- -GCUGCAUC---UC-CGGgAGUC-UG----UCGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 1144 | 0.68 | 0.51862 |
Target: 5'- cCGAagccCGUacAGAGGCUCUCAGAgccCAGCacauugaaUCCa -3' miRNA: 3'- -GCU----GCA--UCUCCGGGAGUCU---GUCG--------AGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 10076 | 0.68 | 0.51862 |
Target: 5'- uGGCGgccAGGCCCUCugGGAUcaGGCUCg -3' miRNA: 3'- gCUGCaucUCCGGGAG--UCUG--UCGAGg -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 22871 | 0.68 | 0.50813 |
Target: 5'- uCGAUcacUGGAGuGCCCUUagcggacucuguAGACAGCUCg -3' miRNA: 3'- -GCUGc--AUCUC-CGGGAG------------UCUGUCGAGg -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 332 | 0.68 | 0.491538 |
Target: 5'- gGGCGagGGAGGCUCggaugccaugcaacCGGGCgAGCUCCu -3' miRNA: 3'- gCUGCa-UCUCCGGGa-------------GUCUG-UCGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 42483 | 0.7 | 0.409046 |
Target: 5'- aGACGUucAGAGGCaUCUCuaAUGGCUCCu -3' miRNA: 3'- gCUGCA--UCUCCG-GGAGucUGUCGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 33929 | 0.7 | 0.390707 |
Target: 5'- gCGACGUGGccugccuGGCCCUguGAgGccguucacccggGCUCCa -3' miRNA: 3'- -GCUGCAUCu------CCGGGAguCUgU------------CGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 39392 | 0.75 | 0.19815 |
Target: 5'- uGAUGUcGAGGCcagggaCCUCAGAgGGCUCUg -3' miRNA: 3'- gCUGCAuCUCCG------GGAGUCUgUCGAGG- -5' |
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13021 | 5' | -56.1 | NC_003390.1 | + | 9946 | 1.13 | 0.000412 |
Target: 5'- gCGACGUAGAGGCCCUCAGACAGCUCCg -3' miRNA: 3'- -GCUGCAUCUCCGGGAGUCUGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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