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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13023 | 3' | -47.9 | NC_003390.1 | + | 26705 | 0.66 | 0.968263 |
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Target: 5'- --gGAACGAUCcuuAGUUgGGCCUUGGg- -3' miRNA: 3'- cgaCUUGUUAG---UCGAaCUGGAACCac -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 2146 | 0.66 | 0.964603 |
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Target: 5'- cGCUGAGCAcaUGGCUaagaGCCUUGGc- -3' miRNA: 3'- -CGACUUGUuaGUCGAac--UGGAACCac -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 45747 | 0.67 | 0.956415 |
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Target: 5'- aGCUGAugugGCAuugccAUCAGCgguggUGGCCagGGUa -3' miRNA: 3'- -CGACU----UGU-----UAGUCGa----ACUGGaaCCAc -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 32673 | 0.67 | 0.941866 |
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Target: 5'- cGCUGAucagGCGGUCAGUcucgGACUUaUGGUa -3' miRNA: 3'- -CGACU----UGUUAGUCGaa--CUGGA-ACCAc -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 27854 | 0.68 | 0.930597 |
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Target: 5'- aGCUGGGCGAUCAGCagaagcaaGACa-UGGUu -3' miRNA: 3'- -CGACUUGUUAGUCGaa------CUGgaACCAc -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 10895 | 0.68 | 0.918054 |
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Target: 5'- aGCgguuGCGAUCAGCcaUUGAUgaUGGUGg -3' miRNA: 3'- -CGacu-UGUUAGUCG--AACUGgaACCAC- -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 40156 | 0.69 | 0.881188 |
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Target: 5'- aGCUGAugauauUAAUCuuguGCUUGACCUccUGaGUGa -3' miRNA: 3'- -CGACUu-----GUUAGu---CGAACUGGA--AC-CAC- -5' |
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| 13023 | 3' | -47.9 | NC_003390.1 | + | 11235 | 1.11 | 0.00343 |
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Target: 5'- uGCUGAACAAUCAGCUUGACCUUGGUGc -3' miRNA: 3'- -CGACUUGUUAGUCGAACUGGAACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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