Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13025 | 3' | -53 | NC_003390.1 | + | 26202 | 0.66 | 0.786307 |
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Target: 5'- cUGCuuGGACUUACCACCG-CGcagAGCauaguuacgaacgaGGCc -3' miRNA: 3'- -ACG--CUUGAAUGGUGGCaGCa--UCG--------------CCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 36319 | 0.66 | 0.779214 |
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Target: 5'- cUGCGAGCUU-CCugUagaUCGUGaacguugacuGCGGCg -3' miRNA: 3'- -ACGCUUGAAuGGugGc--AGCAU----------CGCCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 15038 | 0.68 | 0.704683 |
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Target: 5'- aUGUGAGCUUGCCuACCGguguuggUGUuaagcacgcaugAGUGGUg -3' miRNA: 3'- -ACGCUUGAAUGG-UGGCa------GCA------------UCGCCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 34304 | 0.68 | 0.682541 |
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Target: 5'- aUGgGA--UUACCAgCCGUCugAGCGGCu -3' miRNA: 3'- -ACgCUugAAUGGU-GGCAGcaUCGCCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 3456 | 0.7 | 0.608539 |
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Target: 5'- aGUGGGCUgccaaucggcauugaUGCCACC--UGUAGUGGCc -3' miRNA: 3'- aCGCUUGA---------------AUGGUGGcaGCAUCGCCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 42221 | 0.7 | 0.552914 |
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Target: 5'- gUGCGAAuagucCUUGCCgcccuucccauaauuGCCGUCuuuGCGGCg -3' miRNA: 3'- -ACGCUU-----GAAUGG---------------UGGCAGcauCGCCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 5520 | 0.71 | 0.503145 |
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Target: 5'- gGCGAACUUAgCGuCCGUCaucacacgcugAGCGGUg -3' miRNA: 3'- aCGCUUGAAUgGU-GGCAGca---------UCGCCG- -5' |
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| 13025 | 3' | -53 | NC_003390.1 | + | 15193 | 1.12 | 0.000957 |
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Target: 5'- cUGCGAACUUACCACCGUCGUAGCGGCu -3' miRNA: 3'- -ACGCUUGAAUGGUGGCAGCAUCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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