Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13026 | 5' | -49.8 | NC_003390.1 | + | 19195 | 0.66 | 0.921801 |
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Target: 5'- -cCCUGggACGUCCCuuCGUgCCUGAGu -3' miRNA: 3'- cuGGACa-UGUAGGGu-GUAaGGACUUg -5' |
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| 13026 | 5' | -49.8 | NC_003390.1 | + | 3070 | 0.67 | 0.894034 |
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Target: 5'- aGACCUcUGCAUCCUcuucCAUUCUuUGAACc -3' miRNA: 3'- -CUGGAcAUGUAGGGu---GUAAGG-ACUUG- -5' |
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| 13026 | 5' | -49.8 | NC_003390.1 | + | 5874 | 0.68 | 0.84361 |
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Target: 5'- aGCgUGUGCAagCCCGCcccgUCCUGGAg -3' miRNA: 3'- cUGgACAUGUa-GGGUGua--AGGACUUg -5' |
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| 13026 | 5' | -49.8 | NC_003390.1 | + | 139 | 0.68 | 0.834276 |
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Target: 5'- cGGCCUGUcCAgagaggagaagUCCCugAUUCC-GGGCu -3' miRNA: 3'- -CUGGACAuGU-----------AGGGugUAAGGaCUUG- -5' |
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| 13026 | 5' | -49.8 | NC_003390.1 | + | 44819 | 0.68 | 0.824704 |
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Target: 5'- uGCCUGUGaugUAUCCCACAUcguUgUUGAAUg -3' miRNA: 3'- cUGGACAU---GUAGGGUGUA---AgGACUUG- -5' |
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| 13026 | 5' | -49.8 | NC_003390.1 | + | 37279 | 0.69 | 0.773667 |
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Target: 5'- aGACC---ACAUCCUGCAUuguaUCCUGGAUg -3' miRNA: 3'- -CUGGacaUGUAGGGUGUA----AGGACUUG- -5' |
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| 13026 | 5' | -49.8 | NC_003390.1 | + | 15858 | 1.11 | 0.002015 |
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Target: 5'- gGACCUGUACAUCCCACAUUCCUGAACa -3' miRNA: 3'- -CUGGACAUGUAGGGUGUAAGGACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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