Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13027 | 5' | -59.3 | NC_003390.1 | + | 29513 | 0.66 | 0.437903 |
Target: 5'- gGGAG-CCGGCGGugGaggggGCUcgGGCUuagGCa -3' miRNA: 3'- -CCUCuGGCCGCCugCaa---CGG--UCGG---UG- -5' |
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13027 | 5' | -59.3 | NC_003390.1 | + | 20277 | 0.66 | 0.437903 |
Target: 5'- gGGAG-CCGGCGGugGaggggGCUcgGGCUuagGCa -3' miRNA: 3'- -CCUCuGGCCGCCugCaa---CGG--UCGG---UG- -5' |
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13027 | 5' | -59.3 | NC_003390.1 | + | 19458 | 0.68 | 0.357424 |
Target: 5'- cGGGcuGACUuaGGCGGA---UGCCAGCCAg -3' miRNA: 3'- -CCU--CUGG--CCGCCUgcaACGGUCGGUg -5' |
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13027 | 5' | -59.3 | NC_003390.1 | + | 18591 | 1.12 | 0.00022 |
Target: 5'- aGGAGACCGGCGGACGUUGCCAGCCACa -3' miRNA: 3'- -CCUCUGGCCGCCUGCAACGGUCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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