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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13029 | 3' | -52.8 | NC_003390.1 | + | 38294 | 0.67 | 0.769614 |
Target: 5'- aGGACccuGGGGGccaguaucaccuuGGAGAccUUGAGGGccuUCUGGg -3' miRNA: 3'- -CCUG---UCCCU-------------UCUCU--AGCUCCC---AGAUC- -5' |
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13029 | 3' | -52.8 | NC_003390.1 | + | 40938 | 0.67 | 0.770645 |
Target: 5'- -cACAGGGAAGAGccaacuugGUUaGGGGUgUAGg -3' miRNA: 3'- ccUGUCCCUUCUC--------UAGcUCCCAgAUC- -5' |
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13029 | 3' | -52.8 | NC_003390.1 | + | 35951 | 0.68 | 0.728341 |
Target: 5'- cGGGCAGuccuacGGGGUUGAGGGUCUu- -3' miRNA: 3'- -CCUGUCccuu--CUCUAGCUCCCAGAuc -5' |
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13029 | 3' | -52.8 | NC_003390.1 | + | 28508 | 0.76 | 0.303376 |
Target: 5'- cGGAgGGGGAuccAGGGGUUGuGGGUgUAGg -3' miRNA: 3'- -CCUgUCCCU---UCUCUAGCuCCCAgAUC- -5' |
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13029 | 3' | -52.8 | NC_003390.1 | + | 21938 | 1.03 | 0.004582 |
Target: 5'- aGGACAGGGAAGAG-UCGAGGGUCUAGg -3' miRNA: 3'- -CCUGUCCCUUCUCuAGCUCCCAGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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