Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13030 | 5' | -54.2 | NC_003390.1 | + | 32507 | 0.66 | 0.74302 |
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Target: 5'- aACCCagucacgcggauguCAUCACgcggGCUGaAGGGUgCACCg -3' miRNA: 3'- -UGGG--------------GUAGUGa---CGACcUCUCAaGUGG- -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 38938 | 0.66 | 0.739813 |
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Target: 5'- aGCCCCAcuUCgacaagcgcaugaguGCUGCU-GAGAGUgUCGCa -3' miRNA: 3'- -UGGGGU--AG---------------UGACGAcCUCUCA-AGUGg -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 23478 | 0.66 | 0.73552 |
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Target: 5'- uGCCCCAcgUACUuguccguaacGCUcuaGGAGAcUUCACCu -3' miRNA: 3'- -UGGGGUa-GUGA----------CGA---CCUCUcAAGUGG- -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 35647 | 0.66 | 0.690653 |
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Target: 5'- uGCaCCCAUU--UGCUGGAuaugucuguuaaaGAGUUCGCa -3' miRNA: 3'- -UG-GGGUAGugACGACCU-------------CUCAAGUGg -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 36016 | 0.67 | 0.658271 |
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Target: 5'- aACCCCGUaggACUGCccGGAGA--UCGCUu -3' miRNA: 3'- -UGGGGUAg--UGACGa-CCUCUcaAGUGG- -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 28763 | 0.67 | 0.635798 |
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Target: 5'- gACUCCAaCGCUGUcGGAGAGgacgGCCc -3' miRNA: 3'- -UGGGGUaGUGACGaCCUCUCaag-UGG- -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 6268 | 0.67 | 0.635798 |
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Target: 5'- uACCgCCGgauGCUGgaGGAGGGUgcagacuaugUCGCCa -3' miRNA: 3'- -UGG-GGUag-UGACgaCCUCUCA----------AGUGG- -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 6159 | 0.68 | 0.590891 |
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Target: 5'- uCUCCAUCgaugaugugaaGCUGCUGuucccuGAGAaGUUCACCc -3' miRNA: 3'- uGGGGUAG-----------UGACGAC------CUCU-CAAGUGG- -5' |
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| 13030 | 5' | -54.2 | NC_003390.1 | + | 22624 | 1.12 | 0.000657 |
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Target: 5'- gACCCCAUCACUGCUGGAGAGUUCACCu -3' miRNA: 3'- -UGGGGUAGUGACGACCUCUCAAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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