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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13031 | 3' | -53.5 | NC_003390.1 | + | 44887 | 0.66 | 0.689244 |
Target: 5'- cGAUGUGGGUACaucACaggcauuccagugauGGCAGCGUCa- -3' miRNA: 3'- -UUGUACCCAUGga-UG---------------UCGUCGCAGau -5' |
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13031 | 3' | -53.5 | NC_003390.1 | + | 46002 | 0.66 | 0.673195 |
Target: 5'- cGCAUuaGcGGUACUaACAGCAGCG-CUAg -3' miRNA: 3'- uUGUA--C-CCAUGGaUGUCGUCGCaGAU- -5' |
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13031 | 3' | -53.5 | NC_003390.1 | + | 22985 | 1.04 | 0.001943 |
Target: 5'- cAACAUGGGUACCUACAGCAGCGUCUAc -3' miRNA: 3'- -UUGUACCCAUGGAUGUCGUCGCAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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