Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13031 | 5' | -55.5 | NC_003390.1 | + | 15981 | 0.66 | 0.639256 |
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Target: 5'- aAUGGAGgaacaCUGCUAGGGGaauuCACUCCGc -3' miRNA: 3'- aUGUCUCa----GGCGAUUCCC----GUGAGGUc -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 18703 | 0.66 | 0.639256 |
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Target: 5'- cGCAGAGcaucCCGCUcGGGGUcgaucUUCCAGu -3' miRNA: 3'- aUGUCUCa---GGCGAuUCCCGu----GAGGUC- -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 38243 | 0.66 | 0.6167 |
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Target: 5'- aGCAGGGUCUacaggGCccacAGGGCcUUCCAGg -3' miRNA: 3'- aUGUCUCAGG-----CGau--UCCCGuGAGGUC- -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 41076 | 0.67 | 0.571836 |
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Target: 5'- gGCGGcuGGUagCGUUGGGGGCAUaCCAGg -3' miRNA: 3'- aUGUC--UCAg-GCGAUUCCCGUGaGGUC- -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 22606 | 0.68 | 0.48507 |
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Target: 5'- cACAGAGUUaccaCGCUGuugcGGGgAUUCCAGu -3' miRNA: 3'- aUGUCUCAG----GCGAUu---CCCgUGAGGUC- -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 9869 | 0.69 | 0.434125 |
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Target: 5'- uUGCGGAG-CUGuCUGAGGGC-CUCUAc -3' miRNA: 3'- -AUGUCUCaGGC-GAUUCCCGuGAGGUc -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 27233 | 0.7 | 0.405088 |
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Target: 5'- --aAGAGcCCGCUGAGGaGCAg-CCAGa -3' miRNA: 3'- augUCUCaGGCGAUUCC-CGUgaGGUC- -5' |
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| 13031 | 5' | -55.5 | NC_003390.1 | + | 22950 | 1.06 | 0.000997 |
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Target: 5'- cUACAGAGUCCGCUAAGGGCACUCCAGu -3' miRNA: 3'- -AUGUCUCAGGCGAUUCCCGUGAGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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