Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13033 | 3' | -53.5 | NC_003390.1 | + | 1545 | 0.73 | 0.323222 |
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Target: 5'- cAGC-CGGCCCGGCAGgGGCAGCAc- -3' miRNA: 3'- cUUGcGUCGGGUUGUCgUUGUCGUcg -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 45652 | 0.74 | 0.30707 |
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Target: 5'- ---aGCAGUggAACAGCAGCAGCAGa -3' miRNA: 3'- cuugCGUCGggUUGUCGUUGUCGUCg -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 7480 | 0.74 | 0.291542 |
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Target: 5'- ---aGCuGCUCGGCAGCcucaccccuAGCAGCAGCa -3' miRNA: 3'- cuugCGuCGGGUUGUCG---------UUGUCGUCG- -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 21483 | 0.74 | 0.272283 |
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Target: 5'- aGugGCacaGGCCCAACaacagauggcuuuccAGCAGCAGCAGg -3' miRNA: 3'- cUugCG---UCGGGUUG---------------UCGUUGUCGUCg -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 25824 | 0.75 | 0.262343 |
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Target: 5'- aGAACGCucGUCgAGCA-CAGCAGCAGCu -3' miRNA: 3'- -CUUGCGu-CGGgUUGUcGUUGUCGUCG- -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 501 | 0.75 | 0.255426 |
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Target: 5'- --cCGCuaccaAGCCgAGCAGCGcaaGCAGCAGCu -3' miRNA: 3'- cuuGCG-----UCGGgUUGUCGU---UGUCGUCG- -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 5575 | 0.75 | 0.248658 |
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Target: 5'- --gUGuCAGCCaCAGCAGaGGCAGCAGCg -3' miRNA: 3'- cuuGC-GUCGG-GUUGUCgUUGUCGUCG- -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 40115 | 0.77 | 0.188894 |
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Target: 5'- -cACG-AGUCCAagaGCGGUAACAGCAGCa -3' miRNA: 3'- cuUGCgUCGGGU---UGUCGUUGUCGUCG- -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 42326 | 0.8 | 0.112623 |
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Target: 5'- uGAAUGCAGCCCGccgcaaagACGGCAAUuaugggaagGGCGGCa -3' miRNA: 3'- -CUUGCGUCGGGU--------UGUCGUUG---------UCGUCG- -5' |
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| 13033 | 3' | -53.5 | NC_003390.1 | + | 27034 | 1.13 | 0.000528 |
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Target: 5'- aGAACGCAGCCCAACAGCAACAGCAGCa -3' miRNA: 3'- -CUUGCGUCGGGUUGUCGUUGUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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