Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13035 | 3' | -53.8 | NC_003390.1 | + | 2720 | 0.66 | 0.706257 |
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Target: 5'- aCGAGGGC-CgggGCGGCUaccUCa-CCGAGGa -3' miRNA: 3'- -GCUUCCGaGa--CGUCGA---AGacGGUUCC- -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 14087 | 0.67 | 0.683955 |
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Target: 5'- aGAAGGC-CUGgAGCUUCUcuCCAAa- -3' miRNA: 3'- gCUUCCGaGACgUCGAAGAc-GGUUcc -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 7222 | 0.67 | 0.661444 |
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Target: 5'- gCGGAGGUuucgCUGguGCc---GCCAAGGg -3' miRNA: 3'- -GCUUCCGa---GACguCGaagaCGGUUCC- -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 4430 | 0.68 | 0.638816 |
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Target: 5'- gCGAAGGCgUCUuCAGCUUCcaugaggggGCCAgaccAGGc -3' miRNA: 3'- -GCUUCCG-AGAcGUCGAAGa--------CGGU----UCC- -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 6999 | 0.68 | 0.593568 |
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Target: 5'- uGAGGGCUCUGUacugGGCU-UUGCUAucgauggcauGGGa -3' miRNA: 3'- gCUUCCGAGACG----UCGAaGACGGU----------UCC- -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 27211 | 0.7 | 0.473565 |
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Target: 5'- -aGAGGcCUCUGaggaAGCUcCUGUCGAGGa -3' miRNA: 3'- gcUUCC-GAGACg---UCGAaGACGGUUCC- -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 42459 | 0.71 | 0.442896 |
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Target: 5'- -cGAGGC-CUGcCAGag-CUGCCAGGGg -3' miRNA: 3'- gcUUCCGaGAC-GUCgaaGACGGUUCC- -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 17999 | 0.74 | 0.278823 |
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Target: 5'- uGGAGGUUCUGCAGCUgcucgcgcacgagagCUGCCu--- -3' miRNA: 3'- gCUUCCGAGACGUCGAa--------------GACGGuucc -5' |
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| 13035 | 3' | -53.8 | NC_003390.1 | + | 28459 | 1.1 | 0.000854 |
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Target: 5'- uCGAAGGCUCUGCAGCUUCUGCCAAGGu -3' miRNA: 3'- -GCUUCCGAGACGUCGAAGACGGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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