Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13037 | 5' | -56 | NC_003390.1 | + | 33073 | 0.66 | 0.576355 |
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Target: 5'- --uGCUCCCGuaGGGUGACAGCuguAUAGc- -3' miRNA: 3'- cuuCGGGGGU--UCCACUGUCG---UGUCuu -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 38222 | 0.66 | 0.554005 |
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Target: 5'- aGGGCCuUCCAGGGgccGAUGGUGCAGAc -3' miRNA: 3'- cUUCGG-GGGUUCCa--CUGUCGUGUCUu -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 38356 | 0.67 | 0.510157 |
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Target: 5'- -uGGCCCCCAGGGUccucaaGGCcuGCAGGGu -3' miRNA: 3'- cuUCGGGGGUUCCAcug---UCG--UGUCUU- -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 1448 | 0.68 | 0.457526 |
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Target: 5'- cAGGCCCCgGugcugcucucccAGGUGACgagauacagaGGCACAGGg -3' miRNA: 3'- cUUCGGGGgU------------UCCACUG----------UCGUGUCUu -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 37913 | 0.69 | 0.389073 |
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Target: 5'- aAGGUCCCCAAGGUaacccagggcuGACGGUGCuGAu -3' miRNA: 3'- cUUCGGGGGUUCCA-----------CUGUCGUGuCUu -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 24245 | 0.7 | 0.35309 |
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Target: 5'- cAGGCCCUCAGGGcGGCGGCugAa-- -3' miRNA: 3'- cUUCGGGGGUUCCaCUGUCGugUcuu -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 4960 | 0.71 | 0.281014 |
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Target: 5'- aGGGUCaCCCGAGGUGGCAGCuucCAGc- -3' miRNA: 3'- cUUCGG-GGGUUCCACUGUCGu--GUCuu -5' |
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| 13037 | 5' | -56 | NC_003390.1 | + | 29494 | 1.06 | 0.000854 |
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Target: 5'- uGAAGCCCCCAAGGUGACAGCACAGAAg -3' miRNA: 3'- -CUUCGGGGGUUCCACUGUCGUGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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