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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13041 | 3' | -63.7 | NC_003390.1 | + | 946 | 0.67 | 0.254726 |
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Target: 5'- aGACCGGGCCUaucgcggcuaCCUCaaggAGGCUgCUGc -3' miRNA: 3'- cCUGGCCCGGGg---------GGAGa---UCCGGaGAC- -5' |
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| 13041 | 3' | -63.7 | NC_003390.1 | + | 13148 | 0.69 | 0.182118 |
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Target: 5'- aGGCCGGugcgcuuuacGCUCUCCUCUAGGgcgauguaaccaacCCUCUGa -3' miRNA: 3'- cCUGGCC----------CGGGGGGAGAUCC--------------GGAGAC- -5' |
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| 13041 | 3' | -63.7 | NC_003390.1 | + | 27123 | 0.69 | 0.173832 |
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Target: 5'- aGACUGGGgCCCUCUCUgaugagaccguugAGGCCUg-- -3' miRNA: 3'- cCUGGCCCgGGGGGAGA-------------UCCGGAgac -5' |
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| 13041 | 3' | -63.7 | NC_003390.1 | + | 7713 | 0.7 | 0.137631 |
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Target: 5'- gGGACCcggagcucucGGGCCCUCUgcccAGGUCUCUGg -3' miRNA: 3'- -CCUGG----------CCCGGGGGGaga-UCCGGAGAC- -5' |
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| 13041 | 3' | -63.7 | NC_003390.1 | + | 30713 | 0.72 | 0.105298 |
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Target: 5'- aGugCGGGUuuuaCCCCUa-GGGCCUCUGg -3' miRNA: 3'- cCugGCCCGg---GGGGAgaUCCGGAGAC- -5' |
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| 13041 | 3' | -63.7 | NC_003390.1 | + | 34106 | 1.1 | 0.000117 |
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Target: 5'- cGGACCGGGCCCCCCUCUAGGCCUCUGa -3' miRNA: 3'- -CCUGGCCCGGGGGGAGAUCCGGAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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