Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13042 | 3' | -59.5 | NC_003390.1 | + | 38773 | 0.66 | 0.403789 |
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Target: 5'- uUGGGGUagaUGGUaCCUGAGGGgUCa -3' miRNA: 3'- uACCCCGguaACCA-GGACUUCCgGGa -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 17689 | 0.68 | 0.295442 |
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Target: 5'- gAUGGGaaaccauuacGCCGUgaagacgguaaGGUCCUGAAgGGCCCc -3' miRNA: 3'- -UACCC----------CGGUAa----------CCAGGACUU-CCGGGa -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 29472 | 0.68 | 0.295442 |
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Target: 5'- gGUGGGGCCAcaGGgggCCgUGggGGaUCCg -3' miRNA: 3'- -UACCCCGGUaaCCa--GG-ACuuCC-GGGa -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 35196 | 0.68 | 0.295442 |
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Target: 5'- -aGGGGCCcugUGGaauugCC-GggGGCCCc -3' miRNA: 3'- uaCCCCGGua-ACCa----GGaCuuCCGGGa -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 38302 | 0.7 | 0.216779 |
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Target: 5'- -gGGGGCCAguaucaccUUGGagacCUUGAGGGCCUUc -3' miRNA: 3'- uaCCCCGGU--------AACCa---GGACUUCCGGGA- -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 37842 | 0.71 | 0.174872 |
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Target: 5'- -aGGGuCCugaaGGUCCUGAAGGUCCg -3' miRNA: 3'- uaCCCcGGuaa-CCAGGACUUCCGGGa -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 38058 | 0.76 | 0.075192 |
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Target: 5'- gAUGGGGCUAccGGgCCUGAAGGUCCc -3' miRNA: 3'- -UACCCCGGUaaCCaGGACUUCCGGGa -5' |
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| 13042 | 3' | -59.5 | NC_003390.1 | + | 37879 | 1.05 | 0.000492 |
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Target: 5'- gAUGGGGCCAUUGGUCCUGAAGGCCCUc -3' miRNA: 3'- -UACCCCGGUAACCAGGACUUCCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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