Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13042 | 5' | -55.1 | NC_003390.1 | + | 35962 | 0.66 | 0.691967 |
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Target: 5'- aCGGGGU--UGAGGGUCUUGAucauauagcgGGGaUCa -3' miRNA: 3'- aGUCCCAggACUUCCAGGACU----------UCC-AG- -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 15385 | 0.68 | 0.547372 |
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Target: 5'- gUCuGGcGUCCUGAAGGgCCUaGAcGGUa -3' miRNA: 3'- -AGuCC-CAGGACUUCCaGGA-CUuCCAg -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 40293 | 0.68 | 0.514947 |
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Target: 5'- cCAGGGUacauuuggcCCUGAuGaGUCCUGuagaguuaAAGGUCa -3' miRNA: 3'- aGUCCCA---------GGACUuC-CAGGAC--------UUCCAG- -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 38186 | 0.69 | 0.472996 |
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Target: 5'- uUCcGGGUCCUcAAGGUCCaaAAGGUg -3' miRNA: 3'- -AGuCCCAGGAcUUCCAGGacUUCCAg -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 37868 | 0.72 | 0.333506 |
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Target: 5'- ---uGGUCCUGAAGGcCCUcAAGGUCn -3' miRNA: 3'- agucCCAGGACUUCCaGGAcUUCCAG- -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 38047 | 0.74 | 0.245396 |
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Target: 5'- --cGGG-CCUGAAGGUCCccAGGGUCc -3' miRNA: 3'- aguCCCaGGACUUCCAGGacUUCCAG- -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 38350 | 0.75 | 0.203496 |
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Target: 5'- cCAGGGUCCUcAAGG-CCUGcAGGGUg -3' miRNA: 3'- aGUCCCAGGAcUUCCaGGAC-UUCCAg -5' |
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| 13042 | 5' | -55.1 | NC_003390.1 | + | 37845 | 1.09 | 0.000815 |
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Target: 5'- cUCAGGGUCCUGAAGGUCCUGAAGGUCc -3' miRNA: 3'- -AGUCCCAGGACUUCCAGGACUUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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