Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13043 | 3' | -55.2 | NC_003390.1 | + | 38347 | 0.66 | 0.64623 |
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Target: 5'- -gGGUcCUCAAGG-CCugcAGGGUGAg -3' miRNA: 3'- uaCCGcGAGUUCCuGGuu-UCCCACUg -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 38138 | 0.67 | 0.55599 |
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Target: 5'- cUGGCcCcCAGGGACCuAAaGGUGACa -3' miRNA: 3'- uACCGcGaGUUCCUGGuUUcCCACUG- -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 31825 | 0.67 | 0.533893 |
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Target: 5'- -aGGCGCUCAgcggucuggcgAGcGAUCucAGGGUGGa -3' miRNA: 3'- uaCCGCGAGU-----------UC-CUGGuuUCCCACUg -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 38182 | 0.68 | 0.501365 |
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Target: 5'- -gGGUcCUCAAGGuCCAAAaGGUGAUa -3' miRNA: 3'- uaCCGcGAGUUCCuGGUUUcCCACUG- -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 22815 | 0.69 | 0.449229 |
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Target: 5'- -cGGUGCUCcAGGugagcCCAGAGGG-GAUc -3' miRNA: 3'- uaCCGCGAGuUCCu----GGUUUCCCaCUG- -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 38101 | 0.69 | 0.41942 |
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Target: 5'- -cGGUcCUCAGGGGCUAAAaGGUGAUc -3' miRNA: 3'- uaCCGcGAGUUCCUGGUUUcCCACUG- -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 2169 | 0.7 | 0.409753 |
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Target: 5'- uUGGCaauGCUgcUAAGGGCC--GGGGUGGCc -3' miRNA: 3'- uACCG---CGA--GUUCCUGGuuUCCCACUG- -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 38588 | 0.76 | 0.15945 |
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Target: 5'- -aGGCGCUCcuGGACCAcAGGGaccuccuggagcUGACg -3' miRNA: 3'- uaCCGCGAGuuCCUGGUuUCCC------------ACUG- -5' |
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| 13043 | 3' | -55.2 | NC_003390.1 | + | 37995 | 1.08 | 0.000717 |
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Target: 5'- gAUGGCGCUCAAGGACCAAAGGGUGACa -3' miRNA: 3'- -UACCGCGAGUUCCUGGUUUCCCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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