Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13049 | 3' | -51.6 | NC_003390.1 | + | 1280 | 0.66 | 0.825266 |
Target: 5'- uGGCAGAucaugcucugcgacGUGaCcgACGUUGAGCUGGc -3' miRNA: 3'- gUCGUCU--------------CAC-GuaUGUAGCUCGACUu -5' |
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13049 | 3' | -51.6 | NC_003390.1 | + | 46855 | 0.66 | 0.798266 |
Target: 5'- gGGUAGuGGUGCucuGUAUAagGAGCUGGAg -3' miRNA: 3'- gUCGUC-UCACG---UAUGUagCUCGACUU- -5' |
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13049 | 3' | -51.6 | NC_003390.1 | + | 5584 | 0.7 | 0.590972 |
Target: 5'- aCAGCAGAG-GCAgcaGCGUCcaccuuguugguagGAGCUGGg -3' miRNA: 3'- -GUCGUCUCaCGUa--UGUAG--------------CUCGACUu -5' |
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13049 | 3' | -51.6 | NC_003390.1 | + | 46708 | 1.06 | 0.002417 |
Target: 5'- uCAGCAGAGUGCAUACAUCGAGCUGAAu -3' miRNA: 3'- -GUCGUCUCACGUAUGUAGCUCGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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