Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13050 | 3' | -58.8 | NC_003390.1 | + | 36137 | 0.66 | 0.509936 |
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Target: 5'- gCaAGCCUCCCUCuCCaGCAAgAUCu -3' miRNA: 3'- gGcUCGGAGGGAGcGGgCGUUgUAGc -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 39470 | 0.67 | 0.469813 |
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Target: 5'- -aGAGCCcucugaggUCCCUgGCCU-CGACAUCa -3' miRNA: 3'- ggCUCGG--------AGGGAgCGGGcGUUGUAGc -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 32403 | 0.67 | 0.430426 |
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Target: 5'- aCCGAGgacaagguuggacCCUCCUUC-CaCCGCAuCAUCGa -3' miRNA: 3'- -GGCUC-------------GGAGGGAGcG-GGCGUuGUAGC- -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 31376 | 0.68 | 0.403767 |
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Target: 5'- aUCGAGagugaugcagaCUCCUUCGCCUGCGAgcUCGu -3' miRNA: 3'- -GGCUCg----------GAGGGAGCGGGCGUUguAGC- -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 36367 | 0.68 | 0.386 |
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Target: 5'- -aGAGCgCUCCUaUCGCCCGUu-CGUCu -3' miRNA: 3'- ggCUCG-GAGGG-AGCGGGCGuuGUAGc -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 20204 | 0.7 | 0.308176 |
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Target: 5'- cCCGAGCCcccuccaccgccggcUCCCgCGCCCGCuccGgAUCc -3' miRNA: 3'- -GGCUCGG---------------AGGGaGCGGGCGu--UgUAGc -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 29586 | 0.7 | 0.308176 |
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Target: 5'- cCCGAGCCcccuccaccgccggcUCCCgCGCCCGCuccGgAUCc -3' miRNA: 3'- -GGCUCGG---------------AGGGaGCGGGCGu--UgUAGc -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 36300 | 0.75 | 0.136838 |
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Target: 5'- -aGGGcCCUCCCuUCGCCC-CAGCAUUGg -3' miRNA: 3'- ggCUC-GGAGGG-AGCGGGcGUUGUAGC- -5' |
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| 13050 | 3' | -58.8 | NC_003390.1 | + | 268 | 1.09 | 0.000413 |
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Target: 5'- uCCGAGCCUCCCUCGCCCGCAACAUCGu -3' miRNA: 3'- -GGCUCGGAGGGAGCGGGCGUUGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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