Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13050 | 5' | -49 | NC_003390.1 | + | 40380 | 0.66 | 0.951788 |
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Target: 5'- cACCGGUUGcCGUccAGGCGaauguGGGCGUa -3' miRNA: 3'- -UGGCUAAU-GCA--UUCGCaguu-CUCGCG- -5' |
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| 13050 | 5' | -49 | NC_003390.1 | + | 32881 | 0.68 | 0.905261 |
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Target: 5'- aGCUuGUgcucaGUAuGGCGUCGAGGGUGCa -3' miRNA: 3'- -UGGcUAaug--CAU-UCGCAGUUCUCGCG- -5' |
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| 13050 | 5' | -49 | NC_003390.1 | + | 36358 | 0.69 | 0.839844 |
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Target: 5'- uACCGAggaauagaaUGCGUGAGuCGUCAuccGGGGUGa -3' miRNA: 3'- -UGGCUa--------AUGCAUUC-GCAGU---UCUCGCg -5' |
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| 13050 | 5' | -49 | NC_003390.1 | + | 43695 | 0.7 | 0.790842 |
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Target: 5'- cACCGGggccUGUgGGGCcUCAGGAGCGCu -3' miRNA: 3'- -UGGCUaau-GCA-UUCGcAGUUCUCGCG- -5' |
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| 13050 | 5' | -49 | NC_003390.1 | + | 15738 | 0.72 | 0.714925 |
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Target: 5'- uCCGAUUugGUAcucGGaGUCAAGGGCa- -3' miRNA: 3'- uGGCUAAugCAU---UCgCAGUUCUCGcg -5' |
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| 13050 | 5' | -49 | NC_003390.1 | + | 11852 | 0.73 | 0.669255 |
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Target: 5'- cGCCG-UUAgGUAAGaagaaGUCGGGAGUGUa -3' miRNA: 3'- -UGGCuAAUgCAUUCg----CAGUUCUCGCG- -5' |
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| 13050 | 5' | -49 | NC_003390.1 | + | 305 | 1.13 | 0.002228 |
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Target: 5'- gACCGAUUACGUAAGCGUCAAGAGCGCu -3' miRNA: 3'- -UGGCUAAUGCAUUCGCAGUUCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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