Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13053 | 3' | -55.4 | NC_003390.1 | + | 28139 | 0.66 | 0.629936 |
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Target: 5'- -uCCUGUAcuGUGGCUU---GCACCUCa -3' miRNA: 3'- guGGACAU--CACCGGAuguCGUGGAGa -5' |
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| 13053 | 3' | -55.4 | NC_003390.1 | + | 23884 | 0.66 | 0.61754 |
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Target: 5'- aCACCUGUcgaucaguccgguGGUGGCgCU--AGUACUUCUg -3' miRNA: 3'- -GUGGACA-------------UCACCG-GAugUCGUGGAGA- -5' |
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| 13053 | 3' | -55.4 | NC_003390.1 | + | 2319 | 0.66 | 0.60741 |
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Target: 5'- aACCUugAGUGcuaGCaCUGcCAGCACCUCUg -3' miRNA: 3'- gUGGAcaUCAC---CG-GAU-GUCGUGGAGA- -5' |
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| 13053 | 3' | -55.4 | NC_003390.1 | + | 38341 | 0.68 | 0.529751 |
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Target: 5'- gGCCU-UGGgGGCCaACAGCACCgUCa -3' miRNA: 3'- gUGGAcAUCaCCGGaUGUCGUGG-AGa -5' |
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| 13053 | 3' | -55.4 | NC_003390.1 | + | 37880 | 0.71 | 0.3525 |
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Target: 5'- uGCCU-UGGgGGCCUugAGCACCUg- -3' miRNA: 3'- gUGGAcAUCaCCGGAugUCGUGGAga -5' |
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| 13053 | 3' | -55.4 | NC_003390.1 | + | 43701 | 0.77 | 0.14217 |
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Target: 5'- gGCCUGUGG-GGCCUcaGgAGCGCUUCUa -3' miRNA: 3'- gUGGACAUCaCCGGA--UgUCGUGGAGA- -5' |
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| 13053 | 3' | -55.4 | NC_003390.1 | + | 3481 | 1.07 | 0.000947 |
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Target: 5'- cCACCUGUAGUGGCCUACAGCACCUCUc -3' miRNA: 3'- -GUGGACAUCACCGGAUGUCGUGGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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