Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13057 | 3' | -56.6 | NC_003390.1 | + | 8902 | 0.66 | 0.560727 |
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Target: 5'- --aGAUGCCgAGCuuACGGGCAauaaC-ACCAa -3' miRNA: 3'- uuaCUACGG-UCG--UGCCCGUg---GaUGGU- -5' |
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| 13057 | 3' | -56.6 | NC_003390.1 | + | 1215 | 0.66 | 0.549678 |
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Target: 5'- -cUGAgaGCCucuGUACGGGCuucgGCCUGCUg -3' miRNA: 3'- uuACUa-CGGu--CGUGCCCG----UGGAUGGu -5' |
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| 13057 | 3' | -56.6 | NC_003390.1 | + | 39818 | 0.66 | 0.521289 |
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Target: 5'- cGUGA-GCgGGCacagaaggacuucuuACGGGCACC-ACCAc -3' miRNA: 3'- uUACUaCGgUCG---------------UGCCCGUGGaUGGU- -5' |
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| 13057 | 3' | -56.6 | NC_003390.1 | + | 30156 | 0.67 | 0.474646 |
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Target: 5'- uGAUGGUGCUGGCACcaaauGGGCuCCU-CUAa -3' miRNA: 3'- -UUACUACGGUCGUG-----CCCGuGGAuGGU- -5' |
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| 13057 | 3' | -56.6 | NC_003390.1 | + | 6867 | 0.68 | 0.395685 |
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Target: 5'- ----uUGCCuGCACGGGC-CCUguuaGCCGc -3' miRNA: 3'- uuacuACGGuCGUGCCCGuGGA----UGGU- -5' |
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| 13057 | 3' | -56.6 | NC_003390.1 | + | 7098 | 1.06 | 0.000712 |
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Target: 5'- cAAUGAUGCCAGCACGGGCACCUACCAa -3' miRNA: 3'- -UUACUACGGUCGUGCCCGUGGAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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