Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13058 | 5' | -57.1 | NC_003390.1 | + | 28368 | 0.66 | 0.575662 |
Target: 5'- cGCGaucauccAGGGCcgUCCUGAuGAUGugUCUGu -3' miRNA: 3'- aCGU-------UCCCG--AGGACU-CUGCugAGGCc -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 7639 | 0.68 | 0.452051 |
Target: 5'- gGCAgAGGGC-CC-GAGA--GCUCCGGg -3' miRNA: 3'- aCGU-UCCCGaGGaCUCUgcUGAGGCC- -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 20286 | 0.69 | 0.432669 |
Target: 5'- gGCAuGGaGCUCCUGAGcACuGAUgcgCUGGg -3' miRNA: 3'- aCGUuCC-CGAGGACUC-UG-CUGa--GGCC- -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 7464 | 0.69 | 0.430757 |
Target: 5'- uUGCAGGGGC-CgCUGAGAacacauuaaacACUCUGGa -3' miRNA: 3'- -ACGUUCCCGaG-GACUCUgc---------UGAGGCC- -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 43369 | 0.69 | 0.404527 |
Target: 5'- aGCGAGGGCcuuaCUGAGACGGgaguaguuuauCUCCc- -3' miRNA: 3'- aCGUUCCCGag--GACUCUGCU-----------GAGGcc -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 35237 | 0.71 | 0.289479 |
Target: 5'- aGCAGGGGgaguggugccCUCCUGuGugGAUUgCGGu -3' miRNA: 3'- aCGUUCCC----------GAGGACuCugCUGAgGCC- -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 18086 | 0.74 | 0.196435 |
Target: 5'- gUGCAGuuguaGGCUCCUGAGAaguuCUCCGGc -3' miRNA: 3'- -ACGUUc----CCGAGGACUCUgcu-GAGGCC- -5' |
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13058 | 5' | -57.1 | NC_003390.1 | + | 7412 | 1.1 | 0.00052 |
Target: 5'- cUGCAAGGGCUCCUGAGACGACUCCGGu -3' miRNA: 3'- -ACGUUCCCGAGGACUCUGCUGAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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