Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13061 | 3' | -51 | NC_003390.1 | + | 31006 | 0.66 | 0.87477 |
Target: 5'- -aGCuCUgaaugUGGCGUGGAAGAGguguugGGUg -3' miRNA: 3'- uaCG-GAa----ACUGCGCCUUCUCaa----UCGg -5' |
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13061 | 3' | -51 | NC_003390.1 | + | 17850 | 0.66 | 0.85783 |
Target: 5'- -aGCUgggaUGAUGUGGuAGAGcgGGCCu -3' miRNA: 3'- uaCGGaa--ACUGCGCCuUCUCaaUCGG- -5' |
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13061 | 3' | -51 | NC_003390.1 | + | 2496 | 0.66 | 0.848963 |
Target: 5'- uUGCCUUcUGGCGggugcCGGuGGAGUgguggaAGCCg -3' miRNA: 3'- uACGGAA-ACUGC-----GCCuUCUCAa-----UCGG- -5' |
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13061 | 3' | -51 | NC_003390.1 | + | 24071 | 0.67 | 0.820891 |
Target: 5'- uUGUCgaUGACG-GuGAGGAGUaUGGCCa -3' miRNA: 3'- uACGGaaACUGCgC-CUUCUCA-AUCGG- -5' |
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13061 | 3' | -51 | NC_003390.1 | + | 10663 | 0.98 | 0.01254 |
Target: 5'- gAUGCCUUUGACGCGGAAGAGUauGCCc -3' miRNA: 3'- -UACGGAAACUGCGCCUUCUCAauCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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