Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13063 | 3' | -51.7 | NC_003390.1 | + | 36441 | 0.66 | 0.853706 |
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Target: 5'- ---aCCCCgg--AUGACGACUCaCGCa -3' miRNA: 3'- uuaaGGGGaugaUGCUGUUGAGgGCG- -5' |
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| 13063 | 3' | -51.7 | NC_003390.1 | + | 34154 | 0.67 | 0.816779 |
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Target: 5'- cGUUCCCCcucgaagaGCUcccCGACAGC-CCCGUu -3' miRNA: 3'- uUAAGGGGa-------UGAu--GCUGUUGaGGGCG- -5' |
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| 13063 | 3' | -51.7 | NC_003390.1 | + | 7408 | 0.67 | 0.806994 |
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Target: 5'- ---gCCCCUGCaaggGCuccugaGACGACUCCgGUg -3' miRNA: 3'- uuaaGGGGAUGa---UG------CUGUUGAGGgCG- -5' |
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| 13063 | 3' | -51.7 | NC_003390.1 | + | 20210 | 0.69 | 0.700283 |
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Target: 5'- ---cCCCCUccacCGcCGGCUCCCGCg -3' miRNA: 3'- uuaaGGGGAugauGCuGUUGAGGGCG- -5' |
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| 13063 | 3' | -51.7 | NC_003390.1 | + | 29580 | 0.69 | 0.700283 |
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Target: 5'- ---cCCCCUccacCGcCGGCUCCCGCg -3' miRNA: 3'- uuaaGGGGAugauGCuGUUGAGGGCG- -5' |
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| 13063 | 3' | -51.7 | NC_003390.1 | + | 12387 | 1.09 | 0.001854 |
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Target: 5'- cAAUUCCCCUACUACGACAACUCCCGCc -3' miRNA: 3'- -UUAAGGGGAUGAUGCUGUUGAGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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