Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13065 | 5' | -62.7 | NC_003390.1 | + | 38821 | 0.66 | 0.301871 |
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Target: 5'- -aAAGCCCccucAGCCCCUUCCua-GCCc -3' miRNA: 3'- gaUUCGGGc---UCGGGGGAGGuggCGGc -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 35121 | 0.67 | 0.285621 |
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Target: 5'- -gGGGCCCccggcaauuccacaGGGCCCCUaUUCACCGCa- -3' miRNA: 3'- gaUUCGGG--------------CUCGGGGG-AGGUGGCGgc -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 36620 | 0.67 | 0.280814 |
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Target: 5'- uUGGGCCCGcgaAGCCCCa-CCAagCGCCc -3' miRNA: 3'- gAUUCGGGC---UCGGGGgaGGUg-GCGGc -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 34001 | 0.69 | 0.213532 |
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Target: 5'- -gAGGCCCcuuaGAGCCCCUgCUAgCGCCc -3' miRNA: 3'- gaUUCGGG----CUCGGGGGaGGUgGCGGc -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 1146 | 0.69 | 0.193721 |
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Target: 5'- -gAAGCCCguacagaggcucucaGAGCCCagcacauugaaUCCACCGCCa -3' miRNA: 3'- gaUUCGGG---------------CUCGGGgg---------AGGUGGCGGc -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 37993 | 0.7 | 0.169231 |
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Target: 5'- -cAGGCCCGguAGCCCCaUCagCACCGUCGu -3' miRNA: 3'- gaUUCGGGC--UCGGGGgAG--GUGGCGGC- -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 10106 | 0.72 | 0.113448 |
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Target: 5'- -gGAGCUCGAuGCCCUCUCCAUauCCGg -3' miRNA: 3'- gaUUCGGGCU-CGGGGGAGGUGgcGGC- -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 20198 | 1.09 | 0.000175 |
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Target: 5'- cCUAAGCCCGAGCCCCCUCCACCGCCGg -3' miRNA: 3'- -GAUUCGGGCUCGGGGGAGGUGGCGGC- -5' |
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| 13065 | 5' | -62.7 | NC_003390.1 | + | 29592 | 1.09 | 0.000175 |
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Target: 5'- cCUAAGCCCGAGCCCCCUCCACCGCCGg -3' miRNA: 3'- -GAUUCGGGCUCGGGGGAGGUGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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