Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13066 | 5' | -48.6 | NC_003390.1 | + | 7136 | 0.66 | 0.939452 |
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Target: 5'- aCAAUACccGcGCCCUUggcGGCACCAGc-- -3' miRNA: 3'- -GUUAUGa-CuCGGGAA---UCGUGGUUaga -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 7777 | 0.66 | 0.933241 |
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Target: 5'- gGAUGcCUGAGCagCUUUAGCcuccucgGCCAAUCg -3' miRNA: 3'- gUUAU-GACUCG--GGAAUCG-------UGGUUAGa -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 140 | 0.67 | 0.900872 |
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Target: 5'- aAGUACUG-GCCCUgcagaagGGaCGCCGcgCUg -3' miRNA: 3'- gUUAUGACuCGGGAa------UC-GUGGUuaGA- -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 30454 | 0.67 | 0.900872 |
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Target: 5'- aAGUACUG-GCCCUgcagaagGGaCGCCGcgCUg -3' miRNA: 3'- gUUAUGACuCGGGAa------UC-GUGGUuaGA- -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 42773 | 0.68 | 0.893334 |
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Target: 5'- gCAAUAgaguucuccUUGAGCUUggaGGCACCAAUCa -3' miRNA: 3'- -GUUAU---------GACUCGGGaa-UCGUGGUUAGa -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 5619 | 0.7 | 0.759604 |
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Target: 5'- ---aGCUGGGCCCauaggGGCACgggCAGUCUg -3' miRNA: 3'- guuaUGACUCGGGaa---UCGUG---GUUAGA- -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 6937 | 0.78 | 0.351245 |
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Target: 5'- cCAGUACaGAGCCCUcAGCGgCGGUCUu -3' miRNA: 3'- -GUUAUGaCUCGGGAaUCGUgGUUAGA- -5' |
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| 13066 | 5' | -48.6 | NC_003390.1 | + | 21139 | 1.09 | 0.00333 |
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Target: 5'- cCAAUACUGAGCCCUUAGCACCAAUCUg -3' miRNA: 3'- -GUUAUGACUCGGGAAUCGUGGUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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