Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13067 | 5' | -53.9 | NC_003390.1 | + | 23339 | 0.66 | 0.746251 |
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Target: 5'- cAGACCugucagcCCAGGUCUUUCCguaGCUgCUGu -3' miRNA: 3'- -UCUGGua-----GGUCUAGAGAGGg--UGA-GAC- -5' |
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| 13067 | 5' | -53.9 | NC_003390.1 | + | 18711 | 0.68 | 0.635344 |
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Target: 5'- uGGCCAUCCgcAGAgca-UCCCGCUCg- -3' miRNA: 3'- uCUGGUAGG--UCUagagAGGGUGAGac -5' |
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| 13067 | 5' | -53.9 | NC_003390.1 | + | 10402 | 0.68 | 0.635344 |
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Target: 5'- cAGACCAUCagGGAUCUCaacgUCCUgcuGCUCa- -3' miRNA: 3'- -UCUGGUAGg-UCUAGAG----AGGG---UGAGac -5' |
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| 13067 | 5' | -53.9 | NC_003390.1 | + | 46699 | 0.69 | 0.556776 |
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Target: 5'- cAGuuaCAUCCAGGUCUCgCCCACg--- -3' miRNA: 3'- -UCug-GUAGGUCUAGAGaGGGUGagac -5' |
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| 13067 | 5' | -53.9 | NC_003390.1 | + | 13612 | 0.69 | 0.545735 |
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Target: 5'- aGGGCCcgCUGGuggcCUUUCCUACUCUGu -3' miRNA: 3'- -UCUGGuaGGUCua--GAGAGGGUGAGAC- -5' |
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| 13067 | 5' | -53.9 | NC_003390.1 | + | 39517 | 0.72 | 0.392148 |
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Target: 5'- gAGACCGUUCguacccccgaGGGUCuUCUCCCGC-CUGa -3' miRNA: 3'- -UCUGGUAGG----------UCUAG-AGAGGGUGaGAC- -5' |
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| 13067 | 5' | -53.9 | NC_003390.1 | + | 21727 | 1.08 | 0.001248 |
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Target: 5'- cAGACCAUCCAGAUCUCUCCCACUCUGu -3' miRNA: 3'- -UCUGGUAGGUCUAGAGAGGGUGAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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