Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13069 | 3' | -44.9 | NC_003390.1 | + | 28261 | 0.66 | 0.996219 |
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Target: 5'- -gUCAGCaGC---CUUGUUGGcCUGCAc -3' miRNA: 3'- gaAGUUGaCGuuuGAACAACC-GAUGU- -5' |
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| 13069 | 3' | -44.9 | NC_003390.1 | + | 46023 | 0.66 | 0.996219 |
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Target: 5'- uUUCAACUGCAAACac---GGC-GCAg -3' miRNA: 3'- gAAGUUGACGUUUGaacaaCCGaUGU- -5' |
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| 13069 | 3' | -44.9 | NC_003390.1 | + | 27831 | 0.67 | 0.993594 |
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Target: 5'- -aUCAGCUGCAGGg-UGUcgauacccugGGCUGCu -3' miRNA: 3'- gaAGUUGACGUUUgaACAa---------CCGAUGu -5' |
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| 13069 | 3' | -44.9 | NC_003390.1 | + | 41375 | 0.71 | 0.919565 |
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Target: 5'- --gCAGCcUGCAGGuCUUGgaUGGCUACAa -3' miRNA: 3'- gaaGUUG-ACGUUU-GAACa-ACCGAUGU- -5' |
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| 13069 | 3' | -44.9 | NC_003390.1 | + | 26861 | 0.73 | 0.84603 |
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Target: 5'- -cUCAGCUGCcAGgUUGUUGGCgGCc -3' miRNA: 3'- gaAGUUGACGuUUgAACAACCGaUGu -5' |
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| 13069 | 3' | -44.9 | NC_003390.1 | + | 26793 | 1.11 | 0.006936 |
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Target: 5'- gCUUCAACUGCAAACUUGUUGGCUACAg -3' miRNA: 3'- -GAAGUUGACGUUUGAACAACCGAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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