Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13070 | 5' | -55.5 | NC_003390.1 | + | 38965 | 0.66 | 0.637367 |
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Target: 5'- aUACUUGCGggaggagaacuucucCAGAGGCUUCGACCa- -3' miRNA: 3'- aAUGGGUGCa--------------GUCUCUGGAGCUGGag -5' |
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| 13070 | 5' | -55.5 | NC_003390.1 | + | 9053 | 0.66 | 0.636248 |
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Target: 5'- gUACCCACcuGUgAGAGGCuccaggagccguCUUGGCCUUg -3' miRNA: 3'- aAUGGGUG--CAgUCUCUG------------GAGCUGGAG- -5' |
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| 13070 | 5' | -55.5 | NC_003390.1 | + | 29054 | 0.67 | 0.569346 |
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Target: 5'- -gGCCCG-GUCAGAgauuGACCUgaGCCUCa -3' miRNA: 3'- aaUGGGUgCAGUCU----CUGGAgcUGGAG- -5' |
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| 13070 | 5' | -55.5 | NC_003390.1 | + | 34025 | 0.67 | 0.558328 |
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Target: 5'- -cGCCCGC-UCAGAGGCCUagaggggGGCC-Cg -3' miRNA: 3'- aaUGGGUGcAGUCUCUGGAg------CUGGaG- -5' |
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| 13070 | 5' | -55.5 | NC_003390.1 | + | 10727 | 0.68 | 0.493768 |
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Target: 5'- --uUCCGCGUCAaAGGCaUCGAUCUCg -3' miRNA: 3'- aauGGGUGCAGUcUCUGgAGCUGGAG- -5' |
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| 13070 | 5' | -55.5 | NC_003390.1 | + | 27107 | 1.06 | 0.001173 |
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Target: 5'- cUUACCCACGUCAGAGACCUCGACCUCc -3' miRNA: 3'- -AAUGGGUGCAGUCUCUGGAGCUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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