Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13072 | 5' | -51.9 | NC_003390.1 | + | 22013 | 0.66 | 0.80728 |
Target: 5'- aGGGGUAUGgauUGGCAGAGgugaGAGUUaCGa -3' miRNA: 3'- cUCCUAUAU---ACCGUCUCa---CUCGGaGC- -5' |
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13072 | 5' | -51.9 | NC_003390.1 | + | 34647 | 0.67 | 0.776662 |
Target: 5'- aGAGGAUGcuuuccUAUGGUguugcuaaGGAGUGuGCC-CGa -3' miRNA: 3'- -CUCCUAU------AUACCG--------UCUCACuCGGaGC- -5' |
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13072 | 5' | -51.9 | NC_003390.1 | + | 17853 | 0.81 | 0.150198 |
Target: 5'- uGGGAUgAUGUGGUAGAGcGGGCCUCa -3' miRNA: 3'- cUCCUA-UAUACCGUCUCaCUCGGAGc -5' |
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13072 | 5' | -51.9 | NC_003390.1 | + | 29183 | 1.08 | 0.001858 |
Target: 5'- uGAGGAUAUAUGGCAGAGUGAGCCUCGc -3' miRNA: 3'- -CUCCUAUAUACCGUCUCACUCGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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