Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13078 | 3' | -57.6 | NC_003390.1 | + | 38324 | 0.67 | 0.491297 |
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Target: 5'- aCCuuGAGGGcCuucuGGGCCuuggGGGCCAa -3' miRNA: 3'- gGGugCUCCCaG----UCCGGuua-CCUGGU- -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 21829 | 0.68 | 0.432047 |
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Target: 5'- aCguCGAGGGaCAGGUgGA-GGGCCAg -3' miRNA: 3'- gGguGCUCCCaGUCCGgUUaCCUGGU- -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 31473 | 0.68 | 0.432047 |
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Target: 5'- uUgAUGAGGGcUgGGGcCCAGUGGACUg -3' miRNA: 3'- gGgUGCUCCC-AgUCC-GGUUACCUGGu -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 29460 | 0.69 | 0.385996 |
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Target: 5'- aCCAgcuCGGGaGGUgGGGCCAcaggGGGCCGu -3' miRNA: 3'- gGGU---GCUC-CCAgUCCGGUua--CCUGGU- -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 4447 | 0.69 | 0.377182 |
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Target: 5'- uUCCAUGAGGGggccagacCAGGCCAgaAUGGGg-- -3' miRNA: 3'- -GGGUGCUCCCa-------GUCCGGU--UACCUggu -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 37800 | 0.7 | 0.351565 |
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Target: 5'- aCCuuGAGGGccuUCAGGaCCAAUGGcCCc -3' miRNA: 3'- gGGugCUCCC---AGUCC-GGUUACCuGGu -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 38228 | 0.74 | 0.19668 |
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Target: 5'- gCCCAC-AGGGcCuuccagGGGCCGAUGGugCAg -3' miRNA: 3'- -GGGUGcUCCCaG------UCCGGUUACCugGU- -5' |
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| 13078 | 3' | -57.6 | NC_003390.1 | + | 41242 | 1.1 | 0.000445 |
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Target: 5'- uCCCACGAGGGUCAGGCCAAUGGACCAg -3' miRNA: 3'- -GGGUGCUCCCAGUCCGGUUACCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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