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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13078 | 5' | -55.8 | NC_003390.1 | + | 45081 | 0.67 | 0.618474 |
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Target: 5'- aCUUGGUACCAUCGCCagacacaucgcGAccaucgacagugccaGUCaCAGUGAu -3' miRNA: 3'- -GAGCCAUGGUGGUGG-----------CU---------------CAG-GUCACU- -5' |
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| 13078 | 5' | -55.8 | NC_003390.1 | + | 41550 | 0.67 | 0.592105 |
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Target: 5'- aCUCGGUACCaagGCCACgcaGGG-CCAGa-- -3' miRNA: 3'- -GAGCCAUGG---UGGUGg--CUCaGGUCacu -5' |
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| 13078 | 5' | -55.8 | NC_003390.1 | + | 2796 | 0.67 | 0.570275 |
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Target: 5'- cCUCGGUgagguaGCCGCC-CCGGcccucgucuGUCCAGUc- -3' miRNA: 3'- -GAGCCA------UGGUGGuGGCU---------CAGGUCAcu -5' |
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| 13078 | 5' | -55.8 | NC_003390.1 | + | 1619 | 0.68 | 0.516713 |
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Target: 5'- uUCGGUGCUGCCccuGCCGGG-CCGGcUGc -3' miRNA: 3'- gAGCCAUGGUGG---UGGCUCaGGUC-ACu -5' |
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| 13078 | 5' | -55.8 | NC_003390.1 | + | 41292 | 1.09 | 0.000835 |
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Target: 5'- uCUCGGUACCACCACCGAGUCCAGUGAa -3' miRNA: 3'- -GAGCCAUGGUGGUGGCUCAGGUCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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