miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1308 5' -59.9 NC_001331.1 + 198 0.73 0.024819
Target:  5'- gCGUCCAGCagGGUCGGCUCGauguuguaUUCGCUg -3'
miRNA:   3'- aGUAGGUCG--CCAGCCGGGCa-------GAGCGG- -5'
1308 5' -59.9 NC_001331.1 + 344 0.68 0.060244
Target:  5'- --cUUgGGCGGUCGGCgCCaucggCUUGCCg -3'
miRNA:   3'- aguAGgUCGCCAGCCG-GGca---GAGCGG- -5'
1308 5' -59.9 NC_001331.1 + 3270 0.68 0.064285
Target:  5'- cCAUggGGCGGUCGGCCgCGUgg-GCCg -3'
miRNA:   3'- aGUAggUCGCCAGCCGG-GCAgagCGG- -5'
1308 5' -59.9 NC_001331.1 + 4299 0.66 0.104044
Target:  5'- cCggCCGGCGGcgCGGCCCGcccCUCa-- -3'
miRNA:   3'- aGuaGGUCGCCa-GCCGGGCa--GAGcgg -5'
1308 5' -59.9 NC_001331.1 + 4902 0.68 0.05966
Target:  5'- aCAUCCAGCGcGauaugcacagcugcUUGGUUgGUCUCGCa -3'
miRNA:   3'- aGUAGGUCGC-C--------------AGCCGGgCAGAGCGg -5'
1308 5' -59.9 NC_001331.1 + 5858 1.11 1.3e-05
Target:  5'- gUCAUCCAGCGGUCGGCCCGUCUCGCCc -3'
miRNA:   3'- -AGUAGGUCGCCAGCCGGGCAGAGCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.