Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13082 | 5' | -50.4 | NC_003390.1 | + | 15430 | 0.66 | 0.880659 |
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Target: 5'- uAGCA-CGCuCUUGUauugcgGCGUUGAGAUCu -3' miRNA: 3'- uUCGUgGUGcGAAUA------CGCAACUCUAG- -5' |
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| 13082 | 5' | -50.4 | NC_003390.1 | + | 39093 | 0.66 | 0.872395 |
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Target: 5'- cGGCACCagaccguaACGCUucUGUGCGUgcgcGGUCa -3' miRNA: 3'- uUCGUGG--------UGCGA--AUACGCAacu-CUAG- -5' |
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| 13082 | 5' | -50.4 | NC_003390.1 | + | 3416 | 0.66 | 0.855021 |
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Target: 5'- uGAGUAUCACGCUUGUGU-----GAUCa -3' miRNA: 3'- -UUCGUGGUGCGAAUACGcaacuCUAG- -5' |
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| 13082 | 5' | -50.4 | NC_003390.1 | + | 21154 | 0.67 | 0.845929 |
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Target: 5'- uAGCACCAaucUGCUUG-GCGU--AGAUCu -3' miRNA: 3'- uUCGUGGU---GCGAAUaCGCAacUCUAG- -5' |
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| 13082 | 5' | -50.4 | NC_003390.1 | + | 46920 | 0.76 | 0.322827 |
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Target: 5'- cAGUACCACGCUUAUGCacauugaugGggGAGAUg -3' miRNA: 3'- uUCGUGGUGCGAAUACG---------CaaCUCUAg -5' |
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| 13082 | 5' | -50.4 | NC_003390.1 | + | 44105 | 1.09 | 0.002046 |
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Target: 5'- gAAGCACCACGCUUAUGCGUUGAGAUCa -3' miRNA: 3'- -UUCGUGGUGCGAAUACGCAACUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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