Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13094 | 3' | -57.3 | NC_003401.1 | + | 258 | 0.98 | 0.007709 |
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Target: 5'- cGAGCAAGCAGgCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGUUCGUCgGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 322 | 0.98 | 0.007709 |
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Target: 5'- cGAGCAAGCAGgCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGUUCGUCgGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 132993 | 0.98 | 0.007709 |
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Target: 5'- cGAGCAAGCAGgCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGUUCGUCgGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 133057 | 0.98 | 0.007709 |
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Target: 5'- cGAGCAAGCAGgCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGUUCGUCgGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 362 | 0.99 | 0.006687 |
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Target: 5'- cAAGCcAGCAGCCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGuUCGUCGGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 133097 | 0.99 | 0.006687 |
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Target: 5'- cAAGCcAGCAGCCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGuUCGUCGGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 578 | 1.06 | 0.002079 |
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Target: 5'- cAAGCAAGCAGCCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGUUCGUCGGGAUGACAGGGGAGU- -5' |
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| 13094 | 3' | -57.3 | NC_003401.1 | + | 133293 | 1.06 | 0.002079 |
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Target: 5'- cAAGCAAGCAGCCCUACUGUCCCCUCAg -3' miRNA: 3'- -UUCGUUCGUCGGGAUGACAGGGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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