Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13097 | 5' | -54.8 | NC_003401.1 | + | 30345 | 0.66 | 0.909781 |
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Target: 5'- uUUUGU--UCCCGGgUGCGuaucUAGCGGCg -3' miRNA: 3'- gAGACAagAGGGUCgACGU----GUCGUCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 19137 | 0.67 | 0.868045 |
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Target: 5'- -cCUGgagccCUCCUGGCUGCACGcgucuGuCAGCg -3' miRNA: 3'- gaGACaa---GAGGGUCGACGUGU-----C-GUCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 69321 | 0.67 | 0.86027 |
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Target: 5'- --aUGUUC-CCCAGUUGC-CGGCAcGUc -3' miRNA: 3'- gagACAAGaGGGUCGACGuGUCGU-CG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 122414 | 0.67 | 0.852276 |
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Target: 5'- gUCUGUUCcgCCgcgCAGC-GCGCGGCcGCu -3' miRNA: 3'- gAGACAAGa-GG---GUCGaCGUGUCGuCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 104355 | 0.67 | 0.852276 |
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Target: 5'- gCUCUGUUUUUCUAcCUcGCuCAGCAGUa -3' miRNA: 3'- -GAGACAAGAGGGUcGA-CGuGUCGUCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 8769 | 0.68 | 0.827053 |
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Target: 5'- gUCUGUagcaCCGGCgugagGCAguGCAGCg -3' miRNA: 3'- gAGACAagagGGUCGa----CGUguCGUCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 119793 | 0.68 | 0.800137 |
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Target: 5'- aUCUGg---CCggaGGCgGCGCAGCGGCa -3' miRNA: 3'- gAGACaagaGGg--UCGaCGUGUCGUCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 133616 | 1.11 | 0.001851 |
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Target: 5'- aCUCUGUUCUCCCAGCUGCACAGCAGCc -3' miRNA: 3'- -GAGACAAGAGGGUCGACGUGUCGUCG- -5' |
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| 13097 | 5' | -54.8 | NC_003401.1 | + | 901 | 1.11 | 0.001851 |
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Target: 5'- aCUCUGUUCUCCCAGCUGCACAGCAGCc -3' miRNA: 3'- -GAGACAAGAGGGUCGACGUGUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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