Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13099 | 3' | -56.6 | NC_003401.1 | + | 27282 | 0.66 | 0.86334 |
Target: 5'- aGCGAauacUGAGGCaucGCCGCaacgcGAGGAGAc- -3' miRNA: 3'- -CGCU----GCUUCG---CGGCGga---CUCCUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 72525 | 0.66 | 0.85558 |
Target: 5'- cGCgGACGggGCGCUcag-GAGGAGGc- -3' miRNA: 3'- -CG-CUGCuuCGCGGcggaCUCCUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 93045 | 0.66 | 0.847612 |
Target: 5'- uGCGACGGAGgGggauUCGUCUcGGGAGAu- -3' miRNA: 3'- -CGCUGCUUCgC----GGCGGAcUCCUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 64544 | 0.67 | 0.813815 |
Target: 5'- -aGACGGauGGCG-CGCCUuGAGGAcGAGAu -3' miRNA: 3'- cgCUGCU--UCGCgGCGGA-CUCCU-CUUU- -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 125815 | 0.68 | 0.732765 |
Target: 5'- uCGACGucGCgGCCGUCUccaccgacgaccuuaGAGGAGGGAa -3' miRNA: 3'- cGCUGCuuCG-CGGCGGA---------------CUCCUCUUU- -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 16667 | 0.68 | 0.714764 |
Target: 5'- uGCGGCGcggcuguugugcAAGCgGCCGCCgcacgcgguauacGAGGAGggGc -3' miRNA: 3'- -CGCUGC------------UUCG-CGGCGGa------------CUCCUCuuU- -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 63682 | 0.69 | 0.698565 |
Target: 5'- aGCGGCGGuguucGUGCCGCCUGcaaGAGGc- -3' miRNA: 3'- -CGCUGCUu----CGCGGCGGACuc-CUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 132481 | 0.69 | 0.688365 |
Target: 5'- cGgGGCGGAGgGCCgggaGCCgGGGGAGGc- -3' miRNA: 3'- -CgCUGCUUCgCGG----CGGaCUCCUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 132643 | 0.69 | 0.667829 |
Target: 5'- -gGugGucuccGCGCCGCCggGAGGGGGGc -3' miRNA: 3'- cgCugCuu---CGCGGCGGa-CUCCUCUUu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 82306 | 0.7 | 0.636824 |
Target: 5'- uUGugGAAGCGCCGCUccguccGGAGAu- -3' miRNA: 3'- cGCugCUUCGCGGCGGacu---CCUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 23519 | 0.71 | 0.544437 |
Target: 5'- gGUGACGc-GCGCCGCa-GAGGAGGc- -3' miRNA: 3'- -CGCUGCuuCGCGGCGgaCUCCUCUuu -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 14921 | 0.74 | 0.420337 |
Target: 5'- cGCGACGAGGCgGUCGC-UGAGGGucGAGAg -3' miRNA: 3'- -CGCUGCUUCG-CGGCGgACUCCU--CUUU- -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 100939 | 0.75 | 0.369081 |
Target: 5'- cCGACGAAGCGUCGUCUGAcGAcGAAGg -3' miRNA: 3'- cGCUGCUUCGCGGCGGACUcCU-CUUU- -5' |
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13099 | 3' | -56.6 | NC_003401.1 | + | 11035 | 1.08 | 0.00219 |
Target: 5'- cGCGACGAAGCGCCGCCUGAGGAGAAAa -3' miRNA: 3'- -CGCUGCUUCGCGGCGGACUCCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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