Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 131 | 3' | -52 | AC_000006.1 | + | 6220 | 0.66 | 0.778623 |
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Target: 5'- gCACCACCGUCuuucccgaaUGGAAGUGUGUCg- -3' miRNA: 3'- -GUGGUGGCAGuc-------ACUUUUGCACGGac -5' |
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| 131 | 3' | -52 | AC_000006.1 | + | 16851 | 0.66 | 0.746171 |
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Target: 5'- cCGCCGCCGgcggCGGUucAGGCcgcUGCCUGc -3' miRNA: 3'- -GUGGUGGCa---GUCAcuUUUGc--ACGGAC- -5' |
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| 131 | 3' | -52 | AC_000006.1 | + | 12520 | 0.67 | 0.723865 |
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Target: 5'- uGCCACUGUCGGUGAAGggcGCGaucaGCa-- -3' miRNA: 3'- gUGGUGGCAGUCACUUU---UGCa---CGgac -5' |
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| 131 | 3' | -52 | AC_000006.1 | + | 8169 | 0.68 | 0.654918 |
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Target: 5'- gACC-UCGUagaggcgGGUGAGGGCGUGCUUGa -3' miRNA: 3'- gUGGuGGCAg------UCACUUUUGCACGGAC- -5' |
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| 131 | 3' | -52 | AC_000006.1 | + | 30315 | 0.69 | 0.585087 |
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Target: 5'- gCGCguCCGUCAGcagcaGGAGCGUGCCg- -3' miRNA: 3'- -GUGguGGCAGUCac---UUUUGCACGGac -5' |
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| 131 | 3' | -52 | AC_000006.1 | + | 14878 | 1.07 | 0.001551 |
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Target: 5'- uCACCACCGUCAGUGAAAACGUGCCUGc -3' miRNA: 3'- -GUGGUGGCAGUCACUUUUGCACGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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