Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
131 | 5' | -58.9 | AC_000006.1 | + | 23160 | 0.66 | 0.377273 |
Target: 5'- -aCGCGGGuugggcuCGCaggUGCCGUUGgGCuGGCg -3' miRNA: 3'- uaGUGCCCu------GCG---AUGGCGACgCG-UCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 8313 | 0.66 | 0.359598 |
Target: 5'- uGUCGCGGacGCGCUcCCgGCgGCaGCGGCg -3' miRNA: 3'- -UAGUGCCc-UGCGAuGG-CGaCG-CGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 8271 | 0.67 | 0.334187 |
Target: 5'- -gCGCGGGGCcacgACCGUgccGCGguGCg -3' miRNA: 3'- uaGUGCCCUGcga-UGGCGa--CGCguCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 9285 | 0.67 | 0.334187 |
Target: 5'- --aAUGGGGCGgguccCCGUUGgGCAGCg -3' miRNA: 3'- uagUGCCCUGCgau--GGCGACgCGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 15603 | 0.67 | 0.334187 |
Target: 5'- cGUCACGGGcguGCGCgUGCCcgUGCGCAc- -3' miRNA: 3'- -UAGUGCCC---UGCG-AUGGcgACGCGUcg -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 23315 | 0.67 | 0.334187 |
Target: 5'- cUCGguCGGGACGaga-CGC-GCGCGGCg -3' miRNA: 3'- uAGU--GCCCUGCgaugGCGaCGCGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 8392 | 0.69 | 0.245901 |
Target: 5'- -cCGCGGGGCcggaaccgccGCUGCCGCcgggaGCGCGuccGCg -3' miRNA: 3'- uaGUGCCCUG----------CGAUGGCGa----CGCGU---CG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 16889 | 0.69 | 0.23949 |
Target: 5'- gAUCcCGGGcauaGCUuCCGUUGCGCuGCa -3' miRNA: 3'- -UAGuGCCCug--CGAuGGCGACGCGuCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 13088 | 0.69 | 0.227087 |
Target: 5'- cUCGCGGGcguGCGC-ACCGCcuccugGCGCuuuuGCa -3' miRNA: 3'- uAGUGCCC---UGCGaUGGCGa-----CGCGu---CG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 12101 | 0.69 | 0.221092 |
Target: 5'- gGUgACGGacguGCGCgaaGCCGUgGCGCAGCg -3' miRNA: 3'- -UAgUGCCc---UGCGa--UGGCGaCGCGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 10104 | 0.71 | 0.182815 |
Target: 5'- cUCGC-GGACGCgguuCCagauGUUGCGCAGCg -3' miRNA: 3'- uAGUGcCCUGCGau--GG----CGACGCGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 15409 | 0.74 | 0.098151 |
Target: 5'- -cCAUGGGGCGCcGCCcgagcucuGCUGCGCcGCg -3' miRNA: 3'- uaGUGCCCUGCGaUGG--------CGACGCGuCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 3693 | 0.75 | 0.092629 |
Target: 5'- uGUCAUGGcGGCugcgGCUGCCGCgGCuGCGGCg -3' miRNA: 3'- -UAGUGCC-CUG----CGAUGGCGaCG-CGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 14995 | 0.76 | 0.071235 |
Target: 5'- cUCGCuGGAcucCGCggaUACUGCUGCGCAGCg -3' miRNA: 3'- uAGUGcCCU---GCG---AUGGCGACGCGUCG- -5' |
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131 | 5' | -58.9 | AC_000006.1 | + | 14913 | 1.1 | 0.000178 |
Target: 5'- gAUCACGGGACGCUACCGCUGCGCAGCa -3' miRNA: 3'- -UAGUGCCCUGCGAUGGCGACGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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