Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13100 | 3' | -50.8 | NC_003401.1 | + | 119081 | 0.66 | 0.986011 |
Target: 5'- --cGGCACCGGCGUUaaccgGGUGCGu -3' miRNA: 3'- agaCUGUGGUCGUAGaaca-CUACGCc -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 129390 | 0.66 | 0.986011 |
Target: 5'- uUCgu-CugUAGCGUCUUGUGGaGgGGg -3' miRNA: 3'- -AGacuGugGUCGUAGAACACUaCgCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 107546 | 0.67 | 0.984189 |
Target: 5'- uUUUGAgACgAGCGUCUUGUGuccagaguaguUGCGu -3' miRNA: 3'- -AGACUgUGgUCGUAGAACACu----------ACGCc -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 8888 | 0.67 | 0.982192 |
Target: 5'- gCUGACgcGCCGuCGUCUggacgGUGAggGCGGu -3' miRNA: 3'- aGACUG--UGGUcGUAGAa----CACUa-CGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 127631 | 0.67 | 0.981982 |
Target: 5'- aUCUcGuaaGCCAGCGacaacguUUggGUGAUGCGGg -3' miRNA: 3'- -AGA-Cug-UGGUCGU-------AGaaCACUACGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 63959 | 0.69 | 0.946325 |
Target: 5'- -gUGGCGCgGGCGcgCUcgGUGGUGUGGu -3' miRNA: 3'- agACUGUGgUCGUa-GAa-CACUACGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 82911 | 0.7 | 0.914246 |
Target: 5'- gUUGGCGCCGGUGUUcgGUacacuGGUGCGGu -3' miRNA: 3'- aGACUGUGGUCGUAGaaCA-----CUACGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 87986 | 0.7 | 0.907988 |
Target: 5'- aUUUGGCACgAGUGUCU-GUGGcgggcgUGCGGg -3' miRNA: 3'- -AGACUGUGgUCGUAGAaCACU------ACGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 43318 | 0.73 | 0.823133 |
Target: 5'- gCUGGuCGCCGGCGUUcccGUGAgGCGGg -3' miRNA: 3'- aGACU-GUGGUCGUAGaa-CACUaCGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 46423 | 0.8 | 0.436384 |
Target: 5'- gUCcGGCACCGGCAUCggugcgGUGAUuGCGGu -3' miRNA: 3'- -AGaCUGUGGUCGUAGaa----CACUA-CGCC- -5' |
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13100 | 3' | -50.8 | NC_003401.1 | + | 11284 | 1.12 | 0.004841 |
Target: 5'- cUCUGACACCAGCAUCUUGUGAUGCGGa -3' miRNA: 3'- -AGACUGUGGUCGUAGAACACUACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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