Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13101 | 5' | -56.5 | NC_003401.1 | + | 20716 | 0.66 | 0.88876 |
Target: 5'- -aAUGGAccUGGCGUugaccggccgcCGCCCGCGAu- -3' miRNA: 3'- agUGCCUcuACCGCA-----------GCGGGUGCUug -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 72512 | 0.66 | 0.88876 |
Target: 5'- cUCA-GGAGGaGGCG-CGCCCAguccaGGACc -3' miRNA: 3'- -AGUgCCUCUaCCGCaGCGGGUg----CUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 64529 | 0.66 | 0.886709 |
Target: 5'- -gGCGGuagccaggggagacGGAUGGCG-CGCCUugaGGACg -3' miRNA: 3'- agUGCC--------------UCUACCGCaGCGGGug-CUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 19785 | 0.66 | 0.867352 |
Target: 5'- uUCuCGGGGuc-GCG-CGCCuCGCGAACa -3' miRNA: 3'- -AGuGCCUCuacCGCaGCGG-GUGCUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 68706 | 0.66 | 0.859785 |
Target: 5'- gUUugGGGGcGUGGCGUCGUCUcauauguugcagGCGGc- -3' miRNA: 3'- -AGugCCUC-UACCGCAGCGGG------------UGCUug -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 20610 | 0.67 | 0.827523 |
Target: 5'- aCGCGGGGc---CG-CGCCCugGAACg -3' miRNA: 3'- aGUGCCUCuaccGCaGCGGGugCUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 132437 | 0.67 | 0.818993 |
Target: 5'- gCGCGGgcccGGggGGCGcgcgggggacccUCGCCC-CGGGCg -3' miRNA: 3'- aGUGCC----UCuaCCGC------------AGCGGGuGCUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 54127 | 0.67 | 0.801433 |
Target: 5'- -aACGGGGuuuuUGGCGUCuCCUugGAu- -3' miRNA: 3'- agUGCCUCu---ACCGCAGcGGGugCUug -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 68684 | 0.67 | 0.801433 |
Target: 5'- gCACGGgacguuacAGGUGGCGgC-CCCGCGggUu -3' miRNA: 3'- aGUGCC--------UCUACCGCaGcGGGUGCuuG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 132429 | 0.68 | 0.783258 |
Target: 5'- cCGCGGGGGgccGGgGcCGCCCcCGggUg -3' miRNA: 3'- aGUGCCUCUa--CCgCaGCGGGuGCuuG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 12268 | 0.68 | 0.773963 |
Target: 5'- gUCGCGGAGAaaaccgaaaacGGCcucggCGCCCGCGuGCu -3' miRNA: 3'- -AGUGCCUCUa----------CCGca---GCGGGUGCuUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 132522 | 0.69 | 0.745351 |
Target: 5'- gCGCGGGGcggccgggGGCGgCGgCCGCGGGCc -3' miRNA: 3'- aGUGCCUCua------CCGCaGCgGGUGCUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 11752 | 0.69 | 0.725764 |
Target: 5'- uUCGCGGuGGUGGuCG-CGCCCuuGGGg -3' miRNA: 3'- -AGUGCCuCUACC-GCaGCGGGugCUUg -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 89690 | 0.69 | 0.715845 |
Target: 5'- ---aGGAGGcaggGGCGUCccuggcucaaGCCCAUGGACg -3' miRNA: 3'- agugCCUCUa---CCGCAG----------CGGGUGCUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 118346 | 0.7 | 0.655143 |
Target: 5'- cCGCGGugccgccGccGGgGUCGCCCGCGggUc -3' miRNA: 3'- aGUGCCu------CuaCCgCAGCGGGUGCuuG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 14316 | 0.7 | 0.644911 |
Target: 5'- cUCGCGGAGAcGuGCugcUCGCCaACGAACg -3' miRNA: 3'- -AGUGCCUCUaC-CGc--AGCGGgUGCUUG- -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 15029 | 0.71 | 0.614187 |
Target: 5'- -gGCGGGGcGUGGCGcgCGCgCACGAGu -3' miRNA: 3'- agUGCCUC-UACCGCa-GCGgGUGCUUg -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 88438 | 0.71 | 0.603962 |
Target: 5'- aCAUGGAGGUcGG-GUCGCCCaACGGc- -3' miRNA: 3'- aGUGCCUCUA-CCgCAGCGGG-UGCUug -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 10829 | 0.78 | 0.274921 |
Target: 5'- gUAUGGGGG-GGCGUCGCCUACGGc- -3' miRNA: 3'- aGUGCCUCUaCCGCAGCGGGUGCUug -5' |
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13101 | 5' | -56.5 | NC_003401.1 | + | 13166 | 1.1 | 0.002137 |
Target: 5'- gUCACGGAGAUGGCGUCGCCCACGAACu -3' miRNA: 3'- -AGUGCCUCUACCGCAGCGGGUGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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