Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13102 | 3' | -62.7 | NC_003401.1 | + | 132444 | 0.66 | 0.608099 |
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Target: 5'- gCCCGgCgcgcggGCCCggggGGCGcgCGGGGGa -3' miRNA: 3'- -GGGCgGa-----CGGGga--CCGCaaGCCUCCg -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 132499 | 0.66 | 0.598339 |
|
Target: 5'- gCCGCggGCCCgggccGGCGgggCGGAGGg -3' miRNA: 3'- gGGCGgaCGGGga---CCGCaa-GCCUCCg -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 132534 | 0.66 | 0.627662 |
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Target: 5'- gCCCGgCgcgcgGCgCggGGCGgcCGGGGGCg -3' miRNA: 3'- -GGGCgGa----CGgGgaCCGCaaGCCUCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 132655 | 0.66 | 0.609076 |
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Target: 5'- gCCGCCgggaggggggccgggGCCCggGGCGccUCGGcgGGGCc -3' miRNA: 3'- gGGCGGa--------------CGGGgaCCGCa-AGCC--UCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 132808 | 0.66 | 0.569219 |
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Target: 5'- gCCUGCCUGCUCgCUGGCcugcuugCuGAGGg -3' miRNA: 3'- -GGGCGGACGGG-GACCGcaa----GcCUCCg -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 132828 | 0.66 | 0.584714 |
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Target: 5'- cCCCGCCgaggcgccccggGCCCCggccccccucccGGCGgcgCGGAGa- -3' miRNA: 3'- -GGGCGGa-----------CGGGGa-----------CCGCaa-GCCUCcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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