Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13102 | 5' | -57.7 | NC_003401.1 | + | 132676 | 0.66 | 0.788291 |
Target: 5'- cGGGCCcgGgGGGCGUCgGG-GcGCGGCg -3' miRNA: 3'- -CUCGGa-CgUUUGCAGaCCaC-CGCCGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 12511 | 0.66 | 0.778976 |
Target: 5'- cGAGUCgGCGAagacccGCGUUUcGGUGGUgGGCUg -3' miRNA: 3'- -CUCGGaCGUU------UGCAGA-CCACCG-CCGA- -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 90574 | 0.67 | 0.740467 |
Target: 5'- -uGCCUGCGGAaaccuUCUGaUGGCGGUg -3' miRNA: 3'- cuCGGACGUUUgc---AGACcACCGCCGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 68698 | 0.67 | 0.740467 |
Target: 5'- uGGGCgcauuCUGCGcacggGACGUUacaGGUGGCGGCc -3' miRNA: 3'- -CUCG-----GACGU-----UUGCAGa--CCACCGCCGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 69438 | 0.68 | 0.669721 |
Target: 5'- gGGGCC-GCAGAUGUCgaaccGGUuagugcGGCGGCc -3' miRNA: 3'- -CUCGGaCGUUUGCAGa----CCA------CCGCCGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 22773 | 0.69 | 0.618059 |
Target: 5'- cGAGCacgGCGAGCGUggcgCUGGUGGCaacGCa -3' miRNA: 3'- -CUCGga-CGUUUGCA----GACCACCGc--CGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 35218 | 0.69 | 0.597413 |
Target: 5'- cGGCUcggaacugGCAcaaggAGCGUCUGGUuuuuGGCGGCUg -3' miRNA: 3'- cUCGGa-------CGU-----UUGCAGACCA----CCGCCGA- -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 104101 | 0.7 | 0.546376 |
Target: 5'- -uGCCUGCGAucuCGUCacgGGUGGauuGGCg -3' miRNA: 3'- cuCGGACGUUu--GCAGa--CCACCg--CCGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 98995 | 0.7 | 0.546376 |
Target: 5'- aGGGCCaGCGAGCGUCUGauaGG-GGCUa -3' miRNA: 3'- -CUCGGaCGUUUGCAGACca-CCgCCGA- -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 17328 | 0.71 | 0.516414 |
Target: 5'- -cGCCUGCAAACGUUcaagGGccacGGgGGCUa -3' miRNA: 3'- cuCGGACGUUUGCAGa---CCa---CCgCCGA- -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 120002 | 0.74 | 0.354619 |
Target: 5'- cGGUCUGCcccCGUCccUGGUGGCGGCc -3' miRNA: 3'- cUCGGACGuuuGCAG--ACCACCGCCGa -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 84355 | 0.75 | 0.309207 |
Target: 5'- gGGGUCgUGCGGACGcacGGUGGCGGCUg -3' miRNA: 3'- -CUCGG-ACGUUUGCagaCCACCGCCGA- -5' |
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13102 | 5' | -57.7 | NC_003401.1 | + | 14364 | 1.07 | 0.001816 |
Target: 5'- uGAGCCUGCAAACGUCUGGUGGCGGCUg -3' miRNA: 3'- -CUCGGACGUUUGCAGACCACCGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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